Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GPR119 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal region of the human GPR119 protein (within residues 150-250). [Swiss-Prot Q8TDV5]

Reactivity Notes

Immunogen sequence has 88% identity to rat.

Localization

Cell membrane; Multi-pass membrane protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for GPR119 Antibody [DyLight 405]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: GPR119

GPR119 (G-protein coupled receptor 119) is a class A (rhodopsin-like) 7-transmembrane GPCR with little homology to other receptors, and human GPR119 receptor has 335 amino acids which is highly conserved in mouse and rat. GPR119 is expressed in human insulin-secreting pancreatic islets and incretin (GIP and GLP-1) secreting K- and L-cells of gastrointestinal tract. GPR119 has been suggested to function as a glucose-dependent insulinotropic receptor and its activity is mediated by G proteins which activate adenylate cyclase. Lysophosphatidylcholine (LPC), oleoylethanolamide (OEA), N-acylated ethanolamine phospholipids (NAPEs) and N-oleoyldopamine (OLDA), and 5-hydroxyeicosapentaenoic acid (5-HEPE) have all been proposed as GPR119's endogenous ligands. Moreover, GPR119 agonists have been shown to elicit nutrient-independent incretin and gastrointestinal hormone release as well as nutrient-stimulated insulin secretion, thereby lowering plasma glucose levels during an oral glucose tolerance test. In rodent models, GPR119 mediates improved glucose tolerance and prevents weight gain. GPR119 agonism increases the release of incretin hormones and insulin from enteroendocrine and pancreatic beta cells which may underlie these beneficial effects. GPR119 agonists play a role in reducing food intake and promoting weight loss, possibly through the release of GLP-1, which is known to delay gastric emptying.

Long Name

G Protein-coupled Receptor 119

Alternate Names

GPCR2

Gene Symbol

GPR119

Additional GPR119 Products

Product Documents for GPR119 Antibody [DyLight 405]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GPR119 Antibody [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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