GPR125 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-03561

Novus Biologicals, part of Bio-Techne
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NBP1-03561
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all GPR125 Antibodies »

Product Specifications

Immunogen

Peptide with sequence CTNKSASSFKNS corresponding to internal region according to NP_660333.2.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33754041).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

146 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GPR125 Antibody

Western Blot: GPR125 Antibody [NBP1-03561]

Western Blot: GPR125 Antibody [NBP1-03561]

Western Blot: GPR125 Antibody [NBP1-03561] - Staining of Human Ovary lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: GPR125 Antibody [NBP1-03561]

Immunohistochemistry-Paraffin: GPR125 Antibody [NBP1-03561]

Immunohistochemistry-Paraffin: GPR125 Antibody [NBP1-03561] - Bouin's fixed Human Testis stained with anti-GPR125. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: GPR125 Antibody [NBP1-03561]

Immunohistochemistry-Paraffin: GPR125 Antibody [NBP1-03561]

Immunohistochemistry-Paraffin: GPR125 Antibody [NBP1-03561] - Staining of Human Cortex. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for GPR125 Antibody

Application
Recommended Usage

Immunohistochemistry

3.75 ug/mL

Immunohistochemistry-Paraffin

3.75 ug/mL

Peptide ELISA

Detection limit 1:8000

Western Blot

0.5 - 1.5 ug/mL
Application Notes
WB: Approx. 140 kDa band observed in human ovary lysates (calculated MW of 146 kDa band according to NP_660333.2). An additional band of unknown identity was also consistently observed at 33 kDa. This band was successfully blocked by incubation with the immunizing peptide. GPR125 antibody also validated for IHC-P from a verified customer review.

Reviewed Applications

Read 1 review rated 4 using NBP1-03561 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GPR125

GPR125 is an orphan receptor which has a leucine rich repeat (LRR), an immunoglobulin (Ig) domain, and a hormone-binding domain (HBD). The Ig domain shows similarities to motilin andtitin, while the LRR domain shows similarities to LRIG1 and SLIT1-2. ESTs have been isolated primarily from amnion,connective tissue, ear, embryo, eye,ganglion, heart, lung,placenta, and skin libraries.

Alternate Names

FLJ38547, PGR21, TEM5L

Entrez Gene IDs

166647 (Human)

Gene Symbol

ADGRA3

UniProt

Q8IWK6 (Human)

Additional GPR125 Products

Product Documents for GPR125 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GPR125 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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