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GPR162 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33740

Novus Biologicals, part of Bio-Techne
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NBP2-33740

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: KLLPGRHMLFPPLERVHYLQVPLSRRLSHDETNIFSTPREPGSFLHKWSSSDDIRVLPAQSRALGGPPEYL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GPR162 Antibody

Western Blot: GPR162 Antibody [NBP2-33740]

Western Blot: GPR162 Antibody [NBP2-33740]

Western Blot: GPR162 Antibody [NBP2-33740] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: GPR162 Antibody [NBP2-33740]

Immunocytochemistry/ Immunofluorescence: GPR162 Antibody [NBP2-33740]

Immunocytochemistry/Immunofluorescence: GPR162 Antibody [NBP2-33740] - Staining of human cell line U-2 OS shows localization to microtubule organizing center. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: GPR162 Antibody [NBP2-33740]

Immunohistochemistry-Paraffin: GPR162 Antibody [NBP2-33740]

Immunohistochemistry-Paraffin: GPR162 Antibody [NBP2-33740] - Staining of human pancreas shows low expression as expected.

Applications for GPR162 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GPR162

GPR162, also known as Probable G-protein coupled receptor 162, has a 588 amino acid long isoform that is 64 kDa and a short 304 amino acid isoform that is 33 kDa, located in the cell membrane, mainly expressed in the brain; acts as an orphan receptor. This protein is being studied for its involvement in St. Louis encephalitis, amyotrophic lateral sclerosis, lymphoma, asphyxia neonatorum, dental caries, spotted fever, typhus, lyme disease, toxic shock syndrome, cystic fibrosis, purpura, pancreatic cancer, and pancreatitis. It has been inferred that GPR162 protein is involved in G-protein coupled receptor signaling pathway.

Long Name

G Protein-Coupled Receptor 162

Alternate Names

GRCA

Gene Symbol

GPR162

UniProt

Additional GPR162 Products

Product Documents for GPR162 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GPR162 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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