GPR175 Antibody (6D7)
Novus Biologicals, part of Bio-Techne | Catalog # H00131601-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 6D7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
GPR175 (NP_057456, 281 a.a. ~ 371 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RGFFGSEPKILFSYKCQVDETEEPDVHLPQPYAVARREGLEAAGAAGASAASYSSTQFDSAGGVAYLDDIASMPCHTGSINSTDSERWKAI
Reactivity Notes
Human. Other species not tested.
Localization
Cell Membrane
Specificity
GPR175 - G protein-coupled receptor 175
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for GPR175 Antibody (6D7)
![Western Blot: GPR175 Antibody (6D7) [H00131601-M01]](https://resources.bio-techne.com/images/products/GPR175-Antibody-6D7-Western-Blot-H00131601-M01-img0003.jpg "Western Blot: GPR175 Antibody (6D7) [H00131601-M01]")
Western Blot: GPR175 Antibody (6D7) [H00131601-M01]
Western Blot: GPR175 Antibody (6D7) [H00131601-M01] - Analysis of GPR175 over-expressed 293 cell line, cotransfected with GPR175 Validated Chimera RNAi ( Cat # H00131601-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GPR175 monoclonal antibody (M01), clone 6D7 (Cat # H00131601-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.![Western Blot: GPR175 Antibody (6D7) [H00131601-M01]](https://resources.bio-techne.com/images/products/GPR175-Antibody-6D7-Western-Blot-H00131601-M01-img0004.jpg "Western Blot: GPR175 Antibody (6D7) [H00131601-M01]")
Western Blot: GPR175 Antibody (6D7) [H00131601-M01]
Western Blot: GPR175 Antibody (6D7) [H00131601-M01] - Analysis of GPR175 expression in transfected 293T cell line by GPR175 monoclonal antibody (M01), clone 6D7.Lane 1: GPR175 transfected lysate(41.053 KDa).Lane 2: Non-transfected lysate.Applications for GPR175 Antibody (6D7)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA and RNAi Validation.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: GPR175
Alternate Names
adipocyte associated 1, transmembrane protein, adipocyte asscociated 1
Entrez Gene IDs
131601 (Human)
Gene Symbol
TPRA1
OMIM
608336 (Human)
UniProt
Additional GPR175 Products
Product Documents for GPR175 Antibody (6D7)
Product Specific Notices for GPR175 Antibody (6D7)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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