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GPR27 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14357

Novus Biologicals, part of Bio-Techne
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NBP3-14357

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic 19 amino acid peptide from 3rd cytoplasmic domain of human GPR27.

Epitope

Cytoplasmic Domain

Reactivity Notes

Expected reactivity based on percent sequence identity: Opossum (89%)

Specificity

Human GPR27. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for GPR27 Antibody

Immunohistochemistry-Paraffin: GPR27 Antibody [NBP3-14357]

Immunohistochemistry-Paraffin: GPR27 Antibody [NBP3-14357]

Immunohistochemistry-Paraffin: GPR27 Antibody [NBP3-14357] - Immunohistochemistry of formalin-fixed, paraffin-embedded human prostate after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: GPR27 Antibody [NBP3-14357]

Immunohistochemistry-Paraffin: GPR27 Antibody [NBP3-14357]

Immunohistochemistry-Paraffin: GPR27 Antibody [NBP3-14357] - Immunohistochemistry of formalin-fixed, paraffin-embedded human uterus after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: GPR27 Antibody [NBP3-14357]

Immunohistochemistry-Paraffin: GPR27 Antibody [NBP3-14357]

Immunohistochemistry-Paraffin: GPR27 Antibody [NBP3-14357] - Immunohistochemistry of formalin-fixed, paraffin-embedded human prostate after heat-induced antigen retrieval.

Applications for GPR27 Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 5-12 ug/ml..

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: GPR27

GPR27 is an Orphan-A GPCR with an unknown ligand. GPR27 is a member of the SREB (Super-Conserved Receptor Expressed in Brain) family of GPCRs. GPR27 has been reported to be expressed in several areas of human and rat CNS (Matsumoto et al., 2000), as well as in genital organs. ESTs have been isolated from brain libraries.

Long Name

G Protein-coupled Receptor 27

Alternate Names

SREB1

Gene Symbol

GPR27

Additional GPR27 Products

Product Documents for GPR27 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GPR27 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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