Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.07 mg/ml

View all GPR39 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal region of the human GPR39 protein (within residues 400-500). [Swiss-Prot O43194]

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25604641)

Localization

Cell membrane; Multi-pass membrane protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GPR39 Antibody - BSA Free

Immunohistochemistry: GPR39 Antibody - BSA Free [NLS142]

Immunohistochemistry: GPR39 Antibody - BSA Free [NLS142]

Immunohistochemistry: GPCR GPR39 Antibody [NLS142] - IHC analysis of GPR39 in human brain using Fast Red with hematoxylin counterstain.

Applications for GPR39 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50
Application Notes
This GPR39 antibody is useful for Immunohistochemistry paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.07 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GPR39

GPR39 (G-protein coupled receptor 39) is an orphan GPCR receptor from ghrelin/motilin receptor subfamily of G-protein coupled receptor 1 family, and it is constitutively active with Zn2+ as its physiological agonist. GPR39 is a multipass transmembrane protein found in cell membranes and is expressed in several tissues such as stomach, intestine and hypothalamus. Obestatin, a 23 amino acid peptide originating from ghrelin precursor, was originally shown to be the endogenous ligand of GPR39, a study that happened to be non-reproducible, and later on it was shown that obestatin does not bind, nor activate GPR39 but that Zn2+ could stimulate GPR39 signalling in vitro, thereby inferring that obestatin is not the natural ligand of GPR39. GPR39 receptor also displays a strong ligand-independent signaling activity through G-alpha12/13 as well as G-alphaq. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. GPR39 has been shown to implicate in the regulation of insulin release/pancreatic function, body weight, gastrointestinal mobility, hormone secretion and cell death.

Long Name

G Protein-coupled Receptor 39

Alternate Names

G protein-coupled receptor 39, G-protein coupled receptor 39, MGC149541

Entrez Gene IDs

2863 (Human); 71111 (Mouse)

Gene Symbol

GPR39

UniProt

O43194 (Human), Q5U431 (Mouse)

Additional GPR39 Products

Product Documents for GPR39 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GPR39 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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