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GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25620

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25620

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1864

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fragment of human GPRC5A/RAI3.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free

GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free

Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] -

Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - RAI3 antibody [HL1864] detects RAI3 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. RAI3 stained by RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free

Knockout Validated: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] -

Knockout Validated: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - Unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free

Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] -

Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GPRC5A

GPRC5A is a member of the G protein-coupled receptor family, characterized by a signature 7- transmembrane domain motif. GPRC5A is a lung-specific tumor suppressor and may play a role in the interaction between retinoid acid and G protein signaling pathways. GPRC5A is also involved in embryonic development and epithelial cell differentiation.

Long Name

G Protein-coupled Receptor, Family C, Group 5, Member A

Alternate Names

RAI3, RAIG1

Gene Symbol

GPRC5A

Additional GPRC5A Products

Product Documents for GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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