GRAIL/RNF128 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24610
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Canine, Equine, Primate
Cited:
Human, Mouse
Predicted:
Bovine (94%), Chimpanzee (100%), Monkey (100%), Orangutan (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide corresponding to amino acids portion of amino acids 227-269 of human GRAIL was used as immunogen, GenBank no. NP_919445.1.
Reactivity Notes
The amino acid sequence used as immunogen is 100% homologous in chimpanzee, monkey and orangutan, 94% homologous in cow, 88% homologous in cat and 83% homologous in opossum, rat and mouse.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GRAIL/RNF128 Antibody
Western Blot: GRAIL/RNF128 Antibody [NBP2-24610]
Western Blot: GRAIL/RNF128 Antibody [NBP2-24610] - Bone marrow progenitors were isolated from C57BL/6 mice and incubated with 20ng/ml GM-CSF. (a) Cells were collected at days 0-9. A portion of the cells at day 8 were stimulated with OVA for 24h. Collected cells were processed for protein extraction followed by immunoblot analysis with antibodies to the indicated proteins. Blots were stripped of antibodies prior to probing with the next one. The two braces on the left represent two different gels of the same samples. Signals were quantified and are shown as values under the respective blots. Image collected and cropped by CiteAb from the following publication (hindawi.com/journals/mi/2019/1656484/), licensed under a CC-BY license.Immunohistochemistry-Paraffin: GRAIL/RNF128 Antibody [NBP2-24610]
Immunohistochemistry-Paraffin: GRAIL/RNF128 Antibody [NBP2-24610] - Staining of normal human brain using control (rabbit Ig) at 5 ug/ml.Western Blot: GRAIL/RNF128 Antibody [NBP2-24610]
Western Blot: GRAIL/RNF128 Antibody [NBP2-24610] - Analysis of human GRAIL in human spleen lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse spleen and 4) rat spleen using NBP2-24610 at 1 ug/ml.Applications for GRAIL/RNF128 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
reported in scientific literature (PMID 24356810)
Immunohistochemistry-Paraffin
5-10 ug/ml
Western Blot
0.5-2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GRAIL/RNF128
Long Name
Gene Related to Anergy in Lymphocytes Protein/Ring Finger Protein 128
Alternate Names
RNF128
Gene Symbol
RNF128
UniProt
Additional GRAIL/RNF128 Products
Product Documents for GRAIL/RNF128 Antibody
Product Specific Notices for GRAIL/RNF128 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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