Grancalcin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89785

Novus Biologicals, part of Bio-Techne
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NBP1-89785
NBP1-89785-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Grancalcin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MAYPGYGGGFGNFSIQVPGMQMGQPVPETGPAILLDGYSGPAYSDTYSSAGDSVYTYFSAV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Grancalcin Antibody

Western Blot: Grancalcin Antibody [NBP1-89785]

Western Blot: Grancalcin Antibody [NBP1-89785]

Western Blot: Grancalcin Antibody [NBP1-89785] - Analysis using Anti-GCA antibody NBP1-89785 (A) shows similar pattern to independent antibody NBP1-89786 (B).
Immunohistochemistry-Paraffin: Grancalcin Antibody [NBP1-89785]

Immunohistochemistry-Paraffin: Grancalcin Antibody [NBP1-89785]

Immunohistochemistry-Paraffin: Grancalcin Antibody [NBP1-89785] - Staining in human bone marrow and cerebral cortex tissues using anti-GCA antibody. Corresponding GCA RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Grancalcin Antibody [NBP1-89785]

Immunohistochemistry-Paraffin: Grancalcin Antibody [NBP1-89785]

Immunohistochemistry-Paraffin: Grancalcin Antibody [NBP1-89785] - Staining of human bone marrow, cerebral cortex, kidney and liver using Anti-GCA antibody NBP1-89785 (A) shows similar protein distribution across tissues to independent antibody NBP1-89786 (B).
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Applications for Grancalcin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Grancalcin

Grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation.

Alternate Names

EF-hand calcium-binding protein, grancalcin, EF-hand calcium binding protein, grancalcin, penta-EF-hand protein

Gene Symbol

GCA

Additional Grancalcin Products

Product Documents for Grancalcin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Grancalcin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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