Granzyme B Antibody (OTI4E4) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70870
![Western Blot: Granzyme B Antibody (OTI4E4)Azide and BSA Free [NBP2-70870]](https://resources.bio-techne.com/images/products/Granzyme-B-Antibody-OTI4E4-Azide-and-BSA-Free-Western-Blot-NBP2-70870-img0001.jpg "Western Blot: Granzyme B Antibody (OTI4E4)Azide and BSA Free [NBP2-70870]")
![Immunohistochemistry: Granzyme B Antibody (OTI4E4) - Azide and BSA Free [NBP2-70870]](https://resources.bio-techne.com/images/products/Granzyme-B-Antibody-OTI4E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70870-img0003.jpg "Immunohistochemistry: Granzyme B Antibody (OTI4E4) - Azide and BSA Free [NBP2-70870]")
![Western Blot: Granzyme B Antibody (OTI4E4)Azide and BSA Free [NBP2-70870]](https://resources.bio-techne.com/images/products/Granzyme-B-Antibody-OTI4E4-Azide-and-BSA-Free-Western-Blot-NBP2-70870-img0002.jpg "Western Blot: Granzyme B Antibody (OTI4E4)Azide and BSA Free [NBP2-70870]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI4E4
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 21-247 of human GZMB(NP_004122) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
25.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Granzyme B Antibody (OTI4E4) - Azide and BSA Free
Western Blot: Granzyme B Antibody (OTI4E4)Azide and BSA Free [NBP2-70870]
Western Blot: Granzyme B Antibody (OTI4E4) - Azide and BSA Free [NBP2-70870] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Granzyme B.![Immunocytochemistry/ Immunofluorescence: Granzyme B Antibody (OTI4E4) - Azide and BSA Free [NBP2-70870]](https://resources.bio-techne.com/images/products/Granzyme-B-Antibody-OTI4E4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70870-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Granzyme B Antibody (OTI4E4) - Azide and BSA Free [NBP2-70870]")
Immunocytochemistry/ Immunofluorescence: Granzyme B Antibody (OTI4E4) - Azide and BSA Free [NBP2-70870]
Immunocytochemistry/Immunofluorescence: Granzyme B Antibody (OTI4E4) - Azide and BSA Free [NBP2-70870] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY Granzyme B.![Immunohistochemistry: Granzyme B Antibody (OTI4E4) - Azide and BSA Free [NBP2-70870]](https://resources.bio-techne.com/images/products/Granzyme-B-Antibody-OTI4E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70870-img0004.jpg "Immunohistochemistry: Granzyme B Antibody (OTI4E4) - Azide and BSA Free [NBP2-70870]")
Immunohistochemistry: Granzyme B Antibody (OTI4E4) - Azide and BSA Free [NBP2-70870]
Immunohistochemistry: Granzyme B Antibody (OTI4E4) - Azide and BSA Free [NBP2-70870] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)Applications for Granzyme B Antibody (OTI4E4) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Granzyme B
Granzyme family serine proteases are stored in the granules of cytotoxic T lymphocytes and natural killer cells. Granzymes contain one S1 protease domain and are synthesized as preproproteins. Granzymes are released toward pathogen infected or transformed target cells during cellular immune reactions. Target cell entry by granzymes is through perforin channels. Granzymes trigger apoptosis by caspase-dependent and -independent mechanisms. There are five granzymes (A, B, G, H and K) in human and many more in mouse and rat.
Alternate Names
CGL-1, CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, Granzyme-2, GRB, GrzB, GZMB, HLP, SECT
Gene Symbol
GZMB
Additional Granzyme B Products
Product Documents for Granzyme B Antibody (OTI4E4) - Azide and BSA Free
Product Specific Notices for Granzyme B Antibody (OTI4E4) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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