GRASP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85103

Novus Biologicals, part of Bio-Techne
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NBP1-85103
NBP1-85103-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GRASP1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KESSAVPARSLSSSPQAQPPRPAELSDEEVAELFQRLAETQQEKWMLEEKVKHLEVSSASMAEDLCRKSAIIETYVMDSRIDVSVAAGHTDRSGLGSVLRDLVKPGDENLREMNK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRASP1 Antibody

Immunohistochemistry-Paraffin: GRASP1 Antibody [NBP1-85103]

Immunohistochemistry-Paraffin: GRASP1 Antibody [NBP1-85103]

Immunohistochemistry-Paraffin: GRASP1 Antibody [NBP1-85103] - Staining in human cerebral cortex and skeletal muscle tissues using anti-GRIPAP1 antibody. Corresponding GRIPAP1 RNA-seq data are presented for the same tissues.
Western Blot: GRASP1 Antibody [NBP1-85103]

Western Blot: GRASP1 Antibody [NBP1-85103]

Western Blot: GRASP1 Antibody [NBP1-85103] - Analysis in control (vector only transfected HEK293T lysate) and GRIPAP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: GRASP1 Antibody [NBP1-85103]

Immunocytochemistry/ Immunofluorescence: GRASP1 Antibody [NBP1-85103]

Immunocytochemistry/Immunofluorescence: GRASP1 Antibody [NBP1-85103] - Staining of human cell line A-431 shows localization to cytosol & vesicles. Antibody staining is shown in green.
View 2 more images

Applications for GRASP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRASP1

GRASP1 is a neuron-specific guanine nucleotide exchange factor for the Ras family of small G proteins (RasGEF) and is associated with the GRIP/AMPA receptor complex in brain (Ye et al., 2000 [PubMed 10896157]).[supplied by OMIM]

Long Name

GRIP1 Associated Protein 1

Alternate Names

GRIPAP1

Gene Symbol

GRIPAP1

Additional GRASP1 Products

Product Documents for GRASP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GRASP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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