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GRASP65 Antibody (OTI5G8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72144

Novus Biologicals, part of Bio-Techne
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NBP2-72144
Conjugate
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Key Product Details

Species Reactivity

Human, Rat, Porcine, Canine, Monkey, Primate

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5G8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human GORASP1 (NP_114105) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GRASP65 Antibody (OTI5G8) - Azide and BSA Free

Western Blot: GRASP65 Antibody (OTI5G8)Azide and BSA Free [NBP2-72144]

Western Blot: GRASP65 Antibody (OTI5G8)Azide and BSA Free [NBP2-72144]

Western Blot: GRASP65 Antibody (OTI5G8) - Azide and BSA Free [NBP2-72144] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRASP65 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRASP65.
Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (OTI5G8) - Azide and BSA Free [NBP2-72144]

Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (OTI5G8) - Azide and BSA Free [NBP2-72144]

Immunocytochemistry/Immunofluorescence: GRASP65 Antibody (OTI5G8) - Azide and BSA Free [NBP2-72144] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GRASP65.
Immunohistochemistry: GRASP65 Antibody (OTI5G8) - Azide and BSA Free [NBP2-72144]

Immunohistochemistry: GRASP65 Antibody (OTI5G8) - Azide and BSA Free [NBP2-72144]

Immunohistochemistry: GRASP65 Antibody (OTI5G8) - Azide and BSA Free [NBP2-72144] - Staining of paraffin-embedded Human pancreas tissue using anti-GRASP65 mouse monoclonal antibody.

Applications for GRASP65 Antibody (OTI5G8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:1000-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GRASP65

GRASP65 (Golgi ReAssembly Stacking Protein) is a conserved protein that participates in the stacking of cisternae. This protein associates with GM130 and is phosphorylated by the MEK1 substrate ERK2, Polo-like kinase (Plk) and cdc2-cyclin B. GRASP65 thus plays a role in a cell cycle-dependent reorganization of the Golgi apparatus and may control mammalian cell entry into mitosis.

Alternate Names

Golgi peripheral membrane protein p65, Golgi phosphoprotein 5MGC118897, golgi reassembly stacking protein 1, 65kDa, Golgi reassembly-stacking protein 1, Golgi reassembly-stacking protein of 65 kDa, GRASP65MGC118894, P6565 kDa

Gene Symbol

GORASP1

Additional GRASP65 Products

Product Documents for GRASP65 Antibody (OTI5G8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GRASP65 Antibody (OTI5G8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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