GRASP65 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36526
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 375 to 425 of human Golgi Reassembly Stacking Protein 1, 65kDa using the numbering given in entry NP_114105.1 (GeneID 64689).
Specificity
The epitope recognized by mapping to a region between residue 375 to 425 of human Golgi Reassembly Stacking Protein 1, 65kDa using the numbering given in entry NP_114105.1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GRASP65 Antibody
![Immunoprecipitation: GRASP65 Antibody [NBP2-36526]](https://resources.bio-techne.com/images/products/GRASP65-Antibody-Immunoprecipitation-NBP2-36526-img0001.jpg "Immunoprecipitation: GRASP65 Antibody [NBP2-36526]")
Immunoprecipitation: GRASP65 Antibody [NBP2-36526]
Immunoprecipitation: GRASP65 Antibody [NBP2-36526] - Detection of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GRASP65 antibody NBP2-36526 used for IP at 6 ug per reaction. GRASP65 was also immunoprecipitated by rabbit anti-GRASP65 antibody NBP2-36527. For blotting immunoprecipitated GRASP65, NBP2-36527 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for GRASP65 Antibody
Application
Recommended Usage
Immunoprecipitation
2-10 ul/ml lysate
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
. Validation by IP/Western Blot was performed using a 4-20% SDS-PAGE gel.
. Validation by IP/Western Blot was performed using a 4-20% SDS-PAGE gel.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: GRASP65
Alternate Names
Golgi peripheral membrane protein p65, Golgi phosphoprotein 5MGC118897, golgi reassembly stacking protein 1, 65kDa, Golgi reassembly-stacking protein 1, Golgi reassembly-stacking protein of 65 kDa, GRASP65MGC118894, P6565 kDa
Gene Symbol
GORASP1
UniProt
Additional GRASP65 Products
Product Documents for GRASP65 Antibody
Product Specific Notices for GRASP65 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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