Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all GRASP65 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 390 to 440 of human Golgi Reassembly Stacking Protein 1, 65kDa using the numbering given in entry NP_114105.1.

Specificity

The epitope recognized by mapping to a region between residue 390 to 440 of human Golgi Reassembly Stacking Protein 1, 65kDa using the numbering given in entry NP_114105.1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRASP65 Antibody

Western Blot: GRASP65 Antibody [NBP2-36527]

Western Blot: GRASP65 Antibody [NBP2-36527]

Western Blot: GRASP65 Antibody [NBP2-36527] - Detection of human and mouse GRASP65 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GRASP65 antibody NBP2-36527 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: GRASP65 Antibody [NBP2-36527]

Immunoprecipitation: GRASP65 Antibody [NBP2-36527]

Immunoprecipitation: GRASP65 Antibody [NBP2-36527] - Detection of human GRASP65 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GRASP65 antibody NBP2-36527 used for IP at 6 ug per reaction. GRASP65 was also immunoprecipitated by rabbit anti-GRASP65 antibody NBP2-36526. For blotting immunoprecipitated GRASP65, NBP2-36527 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for GRASP65 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:1000 - 1:5000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-20% SDS-PAGE gel.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GRASP65

GRASP65 (Golgi ReAssembly Stacking Protein) is a conserved protein that participates in the stacking of cisternae. This protein associates with GM130 and is phosphorylated by the MEK1 substrate ERK2, Polo-like kinase (Plk) and cdc2-cyclin B. GRASP65 thus plays a role in a cell cycle-dependent reorganization of the Golgi apparatus and may control mammalian cell entry into mitosis.

Alternate Names

Golgi peripheral membrane protein p65, Golgi phosphoprotein 5MGC118897, golgi reassembly stacking protein 1, 65kDa, Golgi reassembly-stacking protein 1, Golgi reassembly-stacking protein of 65 kDa, GRASP65MGC118894, P6565 kDa

Gene Symbol

GORASP1

UniProt

NP_114105.1 (Human)

Additional GRASP65 Products

Product Documents for GRASP65 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GRASP65 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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