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GREB1 Antibody (CL13859)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24583

Novus Biologicals, part of Bio-Techne
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NBP3-24583-100ul
NBP3-24583-25ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL13859

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for GREB1 Antibody (CL13859)

Immunogen

This antibody was designed to target the recombinant protein GREB1, with the precise immunogen sequence being withheld for proprietary reasons.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for GREB1 Antibody (CL13859)

Immunohistochemistry-Paraffin: GREB1 Antibody (CL13859) [NBP3-24583]

Staining of human ovarian cancer (mucinous) shows strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: GREB1 Antibody (CL13859) [NBP3-24583]

Staining of human fallopian tube shows weak to moderate cytoplasmic and nuclear positivity in glandular cells.

Western Blot: GREB1 Antibody (CL13859) [NBP3-24583]

Western blot analysis in human cell line TD47D.

Applications for GREB1 Antibody (CL13859)

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GREB1

GREB1 is an estrogen-responsive gene that is an early response gene in the estrogen receptor-regulated pathway. It is thought to play an important role in hormone-responsive tissues and cancer. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (a) encodes the longest isoform (a).

Alternate Names

gene regulated by estrogen in breast cancer protein, growth regulation by estrogen in breast cancer 1, KIAA0575Gene regulated in breast cancer 1 protein, protein GREB1

Gene Symbol

GREB1

Additional GREB1 Products

Product Documents for GREB1 Antibody (CL13859)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GREB1 Antibody (CL13859)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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