GREB1 Antibody (CL13863)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24584
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL13863
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for GREB1 Antibody (CL13863)
Immunogen
This antibody was designed to target the recombinant protein GREB1, with the precise immunogen sequence being withheld for proprietary reasons.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for GREB1 Antibody (CL13863)
Immunohistochemistry-Paraffin: GREB1 Antibody (CL13863) [NBP3-24584]
Staining of human small intestine shows no positivity in glandular cells as expected.
Immunocytochemistry/Immunofluorescence: GREB1 Antibody (CL13863) [NBP3-24584]
Staining in U2OS cell line with Anti-GREB1 monoclonal antibody, showing specific staining of nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunohistochemistry-Paraffin: GREB1 Antibody (CL13863) [NBP3-24584]
Staining of human prostate cancer shows strong nuclear positivity in tumor cells.
Applications for GREB1 Antibody (CL13863)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GREB1
Alternate Names
gene regulated by estrogen in breast cancer protein, growth regulation by estrogen in breast cancer 1, KIAA0575Gene regulated in breast cancer 1 protein, protein GREB1
Gene Symbol
GREB1
Additional GREB1 Products
Product Documents for GREB1 Antibody (CL13863)
Product Specific Notices for GREB1 Antibody (CL13863)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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