GRF2 Antibody (3D10)
Novus Biologicals, part of Bio-Techne | Catalog # H00002889-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3D10
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
RAPGEF1 (AAH41710, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGNAIEKQKPLKRSHLYPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSW
Specificity
RAPGEF1 - Rap guanine nucleotide exchange factor (GEF) 1
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for GRF2 Antibody (3D10)
![Western Blot: GRF2 Antibody (3D10) [H00002889-M01]](https://resources.bio-techne.com/images/products/GRF2-Antibody-3D10-Western-Blot-H00002889-M01-img0003.jpg "Western Blot: GRF2 Antibody (3D10) [H00002889-M01]")
Western Blot: GRF2 Antibody (3D10) [H00002889-M01]
Western Blot: GRF2 Antibody (3D10) [H00002889-M01] - Analysis of RAPGEF1 expression in transfected 293T cell line by RAPGEF1 monoclonal antibody (M01), clone 3D10.Lane 1: RAPGEF1 transfected lysate(122.7 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: GRF2 Antibody (3D10) [H00002889-M01] - Detection limit for recombinant GST tagged RAPGEF1 is approximately 0.1ng/ml as a capture antibody.
Applications for GRF2 Antibody (3D10)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: GRF2
Alternate Names
C3GGRF2guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene), CRK SH3-binding GNRP, DKFZp781P1719, Guanine nucleotide-releasing factor 2, Protein C3G, Rap guanine nucleotide exchange factor (GEF) 1, rap guanine nucleotide exchange factor 1
Entrez Gene IDs
2889 (Human)
Gene Symbol
RAPGEF1
OMIM
600303 (Human)
UniProt
Additional GRF2 Products
Product Documents for GRF2 Antibody (3D10)
Product Specific Notices for GRF2 Antibody (3D10)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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