GRF2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88266

Novus Biologicals, part of Bio-Techne
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NBP1-88266
NBP1-88266-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GRF2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRF2 Antibody

Western Blot: GRF2 Antibody [NBP1-88266]

Western Blot: GRF2 Antibody [NBP1-88266]

Western Blot: GRF2 Antibody [NBP1-88266] - Analysis in control (vector only transfected HEK293T lysate) and RAPGEF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: GRF2 Antibody [NBP1-88266]

Immunohistochemistry-Paraffin: GRF2 Antibody [NBP1-88266]

Immunohistochemistry-Paraffin: GRF2 Antibody [NBP1-88266] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: GRF2 Antibody [NBP1-88266]

Immunohistochemistry-Paraffin: GRF2 Antibody [NBP1-88266]

Immunohistochemistry-Paraffin: GRF2 Antibody [NBP1-88266] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
View 1 more images

Applications for GRF2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRF2

GRF2 encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq]

Alternate Names

C3GGRF2guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene), CRK SH3-binding GNRP, DKFZp781P1719, Guanine nucleotide-releasing factor 2, Protein C3G, Rap guanine nucleotide exchange factor (GEF) 1, rap guanine nucleotide exchange factor 1

Gene Symbol

RAPGEF1

Additional GRF2 Products

Product Documents for GRF2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GRF2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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