GRK5 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-04998

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GRK5 Antibodies »

Product Summary for GRK5 Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 371-590 of human GRK5 (NP_005299.1). LGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSHKFSEEAKSICKMLLTKDAKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDHTDDDFYSKFSTGSVSIPWQNEMIETECFKELNVFGPNGTLPPDLNRNHPPEPPKKGLLQRLFKRQHQNNSKSSPSSKTSFNHHINSNHVSSNSTGSS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GRK5 Antibody - BSA Free

Western Blot: GRK5 AntibodyBSA Free [NBP3-04998]

Western Blot: GRK5 AntibodyBSA Free [NBP3-04998]

Western Blot: GRK5 Antibody [NBP3-04998] - Analysis of extracts of various cell lines, using GRK5 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit
Immunocytochemistry/ Immunofluorescence: GRK5 Antibody - BSA Free [NBP3-04998]

Immunocytochemistry/ Immunofluorescence: GRK5 Antibody - BSA Free [NBP3-04998]

Immunocytochemistry/Immunofluorescence: GRK5 Antibody [NBP3-04998] - Analysis of L929 cells using GRK5 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. GRK5 antibody
Immunohistochemistry-Paraffin: GRK5 Antibody - BSA Free [NBP3-04998]

Immunohistochemistry-Paraffin: GRK5 Antibody - BSA Free [NBP3-04998]

Immunohistochemistry-Paraffin: GRK5 Antibody [NBP3-04998] - Paraffin-embedded mouse heart using GRK5 antibody at dilution of 1:100 (40x lens).
View 2 more images

Applications for GRK5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GRK5

GRK5 is a member of the guanine nucleotide binding protein (G protein)coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein coupled receptors thus initiating their deactivation. This GRCR GRK signaling system regulates many physiological processes including transcription, cell movement, and apoptosis.

Long Name

G protein-coupled Receptor Kinase 5

Alternate Names

GPRK5

Gene Symbol

GRK5

Additional GRK5 Products

Product Documents for GRK5 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GRK5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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