Growth Hormone R Antibody (10C11)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26554
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10C11
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Growth Hormone R Antibody (10C11)
Immunogen
A synthesized peptide derived from Human Growth Hormone R [UniProt P10912]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Growth Hormone R Antibody (10C11)
Immunohistochemistry: Growth Hormone R Antibody (10C11) [NBP3-26554] -
Immunohistochemistry: Growth Hormone R Antibody (10C11) [NBP3-26554] - Image of Growth Hormone R Antibody (10C11) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: Growth Hormone R Antibody (10C11) [NBP3-26554] -
Immunohistochemistry: Growth Hormone R Antibody (10C11) [NBP3-26554] - Image of Growth Hormone R Antibody (10C11) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunocytochemistry/Immunofluorescence: Growth Hormone R Antibody (10C11) [NBP3-26554] -
Immunocytochemistry/Immunofluorescence: Growth Hormone R Antibody (10C11) [NBP3-26554] - Staining of HepG2 Cells with Growth Hormone R Antibody (10C11) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).Applications for Growth Hormone R Antibody (10C11)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Immunohistochemistry
1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Growth Hormone R/GHR
Long Name
Growth Hormone Receptor
Alternate Names
GHR
Gene Symbol
GHR
Additional Growth Hormone R/GHR Products
Product Documents for Growth Hormone R Antibody (10C11)
Product Specific Notices for Growth Hormone R Antibody (10C11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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