GRP75/HSPA9B/Mortalin Antibody (6-A9-R)

Western Blot: GRP75/HSPA9B/Mortalin Antibody (6-A9-R) [NBP3-32407]

Western Blot: GRP75/HSPA9B/Mortalin Antibody (6-A9-R) [NBP3-32407] - Western blot analysis of GRP75/HSPA9B/Mortalin on different lysates with Mouse anti-GRP75/HSPA9B/Mortalin antibody (NBP3-32407) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: Mouse liver tissue lysate
Lane 7: Rat liver tissue lysate
Lane 8: Mouse kidney tissue lysate
Lane 9: Rat kidney tissue lysate

Predicted band size: 74 kDa
Observed band size: 74 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32407) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: GRP75/HSPA9B/Mortalin Antibody (6-A9-R) [NBP3-32407]

Immunohistochemistry: GRP75/HSPA9B/Mortalin Antibody (6-A9-R) [NBP3-32407] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-GRP75/HSPA9B/Mortalin antibody (NBP3-32407) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32407) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody (6-A9-R) [NBP3-32407]

Immunocytochemistry/ Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody (6-A9-R) [NBP3-32407] - Immunocytochemistry analysis of K-562 cells labeling GRP75/HSPA9B/Mortalin with Mouse anti-GRP75/HSPA9B/Mortalin antibody (NBP3-32407) at 1/100 dilution.

Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-GRP75/HSPA9B/Mortalin antibody (NBP3-32407) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.