GRP75/HSPA9B/Mortalin Antibody (9D6H0)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15365

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15365-100ul
NBP3-15365-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9D6H0

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GRP75/HSPA9B Antibodies »

Product Summary for GRP75/HSPA9B/Mortalin Antibody (9D6H0)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 580-679 of human GRP75/HSPA9B/Mortalin (P38646). EAVNMAEGIIHDTETKMEEFKDQLPADECNKLKEEISKMRELLARKDSETGENIRQAASSLQQASLKLFEMAYKKMASEREGSGSSGTGEQKEDQKEEKQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRP75/HSPA9B/Mortalin Antibody (9D6H0)

Western Blot: GRP75/HSPA9B/Mortalin Antibody (9D6H0) [NBP3-15365]

Western Blot: GRP75/HSPA9B/Mortalin Antibody (9D6H0) [NBP3-15365]

Western Blot: GRP75/HSPA9B/Mortalin Antibody (9D6H0) [NBP3-15365] - Western blot analysis of extracts of various cell lines, using GRP75/HSPA9B/Mortalin Rabbit mAb (NBP3-15365) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Applications for GRP75/HSPA9B/Mortalin Antibody (9D6H0)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GRP75/HSPA9B

The HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains. HSC 70, GRP 75 and GRP 78 are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70, which are found in both the cytosol and nucleus of mammalian cells, play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery. They are involved in chaperoning nascent polypeptide chains and in protecting cells against the accumulation of improperly folded proteins. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. Research indicates that members of the HSP 70 family may act as force-generating motors, relying on the hydrolysis of ATP for their activity.

Long Name

75 KD Glucose Regulated Protein

Alternate Names

CSA, HSPA9, HSPA9B, Mortalin-2, MOT2, PBP74

Gene Symbol

HSPA9

Additional GRP75/HSPA9B Products

Product Documents for GRP75/HSPA9B/Mortalin Antibody (9D6H0)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GRP75/HSPA9B/Mortalin Antibody (9D6H0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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