GSDMDC1 Antibody (PD00-18) - N-terminal

Western Blot: GSDMDC1 Antibody (PD00-18) - N-terminal [NBP3-32369]

Western Blot: GSDMDC1 Antibody (PD00-18) - N-terminal [NBP3-32369] - Western blot analysis of GSDMDC1 on different lysates with Rabbit anti-GSDMDC1 antibody (NBP3-32369) at 1/1,000 dilution.

Lane 1: SiHa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: THP-1 cell lysate
Lane 4: PC-3M cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: L6 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 53/30 kDa
Observed band size: 53/30 kDa

Exposure time: 43 seconds; ECL;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32369) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.