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GSG2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-26626

Novus Biologicals, part of Bio-Techne
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NBP1-26626

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 300 and 350 of human serine/threonine-protein kinase Haspin (germ cell-specific gene 2) using the numbering given in entry NP_114171.2 (GeneID 83903).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GSG2 Antibody

Western Blot: GSG2 Antibody [NBP1-26626]

Western Blot: GSG2 Antibody [NBP1-26626]

Western Blot: GSG2 Antibody [NBP1-26626] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Haspin antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 10ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Immunohistochemistry-Paraffin: GSG2 Antibody [NBP1-26626]

Immunohistochemistry-Paraffin: GSG2 Antibody [NBP1-26626]

Immunohistochemistry-Paraffin: GSG2 Antibody [NBP1-26626] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Haspin used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for GSG2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

5-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GSG2

Hapsin is a serine-threonine protein kinase that was originally identified as a cDNA expressed specifically in mouse testicular germ cells. Haspin associates with the chromosomes, centrosomes, and spindle and is critical for proper chromosome alignment and segregation. Haspin is responsible for the phosphorylation of histone H3 at threonine 3.

Alternate Names

EC 2.7.11.1, germ cell associated 2 (haspin), Germ cell-specific gene 2 protein, Haploid germ cell-specific nuclear protein kinase, HASPIN, H-haspin, serine/threonine-protein kinase haspin

Entrez Gene IDs

83903 (Human)

Gene Symbol

HASPIN

UniProt

Additional GSG2 Products

Product Documents for GSG2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GSG2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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