GSK-3 beta Antibody (3D6B4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52478

Novus Biologicals, part of Bio-Techne
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NBP2-52478-0.025mg
NBP2-52478
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3D6B4

Format

BSA Free

Concentration

1 mg/ml

View all GSK-3 beta Antibodies »

Product Summary for GSK-3 beta Antibody (3D6B4) - BSA Free

Immunogen

Purified recombinant fragment of human GSK-3 beta (AA: 2-159) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GSK-3 beta Antibody (3D6B4) - BSA Free

Western Blot: GSK-3 beta Antibody (3D6B4)BSA Free [NBP2-52478]

Western Blot: GSK-3 beta Antibody (3D6B4)BSA Free [NBP2-52478]

Western Blot: GSK-3 beta Antibody (3D6B4) [NBP2-52478] - Analysis using GSK3B mAb against human GSK3B (AA: 2-159) recombinant protein. (Expected MW is 20.3 kDa)
Immunohistochemistry-Paraffin: GSK-3 beta Antibody (3D6B4) - BSA Free [NBP2-52478]

Immunohistochemistry-Paraffin: GSK-3 beta Antibody (3D6B4) - BSA Free [NBP2-52478]

Immunohistochemistry-Paraffin: GSK-3 beta Antibody (3D6B4) [NBP2-52478] - Analysis of esophageal cancer tissues using GSK3B mouse mAb with DAB staining.
Flow Cytometry: GSK-3 beta Antibody (3D6B4) - BSA Free [NBP2-52478]

Flow Cytometry: GSK-3 beta Antibody (3D6B4) - BSA Free [NBP2-52478]

Flow Cytometry: GSK-3 beta Antibody (3D6B4) [NBP2-52478] - Analysis of NIH/3T3 cells using GSK3B mouse mAb (green) and negative control (red).
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Applications for GSK-3 beta Antibody (3D6B4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunohistochemistry

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GSK-3 beta

Glycogen synthase kinase 3beta (GSK-3 beta) is a Serine/Threonine protein kinas involved in the insulin and wingless pathways (1-2). Originally identified as a regulator of glycogen synthase, it has been shown to regulate a diverse array of cellular functions. GSK-3 beta activity is down regulated by phosphorylation on Serine 9 by PKB, or activated by phosphorylation on Tyrosine 216. Once activated, the protein can phosphorylate p53, c-jun, heat shock factor-1 and cyclin D1 (3-4). It is also known to phosphorylate Tau in Allzheimer's disease. Additionally, increased GSK-3 beta protein levels are found in Alzheimer's disease brains.

Long Name

Glycogen Synthase Kinase 3 beta

Alternate Names

GSK3 beta, GSK3B

Gene Symbol

GSK3B

Additional GSK-3 beta Products

Product Documents for GSK-3 beta Antibody (3D6B4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GSK-3 beta Antibody (3D6B4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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