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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunohistochemistry, Immunoprecipitation, SDS-Page, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 24E9.G9.G8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This Protein A purified GSTZ1 Antibody (24E9.G9.G8) was prepared by repeated immunizations in mice with GSTGSTZ1 Protein (Uniprot: O43708)

Reactivity Notes

Cross reactivity from other sources has not been determined

Specificity

Reacts specifically with GSTZ1 protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

This product is purified from roller bottle culture by Protein A chromatography followed by extensive dialysis against the buffer stated above

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for GSTZ1 Antibody (24E9.G9.G8)

SDS-PAGE: GSTZ1 Antibody (24E9.G9.G8) [NBP2-44177]

SDS-PAGE: GSTZ1 Antibody (24E9.G9.G8) [NBP2-44177]

SDS-Page: GSTZ1 Antibody (24E9.G9.G8) [NBP2-44177] - Lane 1: Reduced Mouse anti-GSTZ1 Monoclonal Antibody. Lane 2: 3 UL OPAL Pre-stained Marker. Lane 3: Non-reduced Mouse anti-GSTZ1 Monoclonal Antibody. Load: 1 Ug per lane. Predicted/Observed size: Non-reduced at 160 kDa/observed at 180-200 kDa; Reduced at 55, 25 kDa. Non-reduced migrates at slightly higher molecular weight.
GSTZ1 Antibody (24E9.G9.G8)

GSTZ1 Antibody (24E9.G9.G8)

Western Blot of Mouse anti-GSTZ1 Monoclonal Antibody. Lane 1: Recombinant GSTZ1 protein. Lane 2: GST

Applications for GSTZ1 Antibody (24E9.G9.G8)

Application
Recommended Usage

ELISA

1:50000-1:200000

Immunohistochemistry

1:10-1:500

Immunoprecipitation

1:10-1:500

SDS-Page

1:100-1:2000

Western Blot

1:500-1:1000
Application Notes
This product has been tested by ELISA, SDS-Page, and Western Blot. Suitable for most immunological techniques requiring high titer binding and lot-to-lot consistency. Specific conditions for reactivity should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: GSTZ1

GSTZ1 is a member of the glutathione S-transferase (GSTs) super-family which encodes multifunctional enzymes important in the detoxification of electrophilic molecules, including carcinogens, mutagens, and several therapeutic drugs, by conjugation with glutathione. This enzyme also plays a significant role in the catabolism of phenylalanine and tyrosine. Thus defects in this enzyme may lead to severe metabolic disorders including alkaptonuria, phenylketonuria and tyrosinaemia. Several transcript variants of this gene encode multiple protein isoforms.

Alternate Names

EC 2.5.1.18, EC 5.2.1.2, glutathione S-alkyltransferase, glutathione S-aralkyltransferase, Glutathione S-transferase zeta 1, glutathione transferase zeta 1, GSTZ1-1maleylacetoacetate isomerase, MAAIglutathione S-aryltransferase, MAI, maleylacetone isomerase, MGC2029, S-(hydroxyalkyl)glutathione lyase

Gene Symbol

GSTZ1

UniProt

Additional GSTZ1 Products

Product Documents for GSTZ1 Antibody (24E9.G9.G8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GSTZ1 Antibody (24E9.G9.G8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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