GTF2H2 Antibody (PCRP-GTF2H2-1B9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07348

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PCRP-GTF2H2-1B9

Concentration

0.2 mg/ml

View all GTF2H2 Antibodies »

Product Summary for GTF2H2 Antibody (PCRP-GTF2H2-1B9)

Immunogen

Recombinant full-length human GTF2H2 protein (Uniprot: Q13888)

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08257)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for GTF2H2 Antibody (PCRP-GTF2H2-1B9)

Immunocytochemistry/ Immunofluorescence: GTF2H2 Antibody (PCRP-GTF2H2-1B9) [NBP3-07348]

Immunocytochemistry/ Immunofluorescence: GTF2H2 Antibody (PCRP-GTF2H2-1B9) [NBP3-07348]

Immunocytochemistry/Immunofluorescence: GTF2H2 Antibody (PCRP-GTF2H2-1B9) [NBP3-07348] - Immunofluorescence Analysis of PFA-fixed MCF7 cells. GTF2H2 Mouse Monoclonal Antibody (PCRP-GTF2H2-1B9) followed by goat anti- Mouse IgG-CF488 (green). Phalloidin counterstain (red).
Flow Cytometry: GTF2H2 Antibody (PCRP-GTF2H2-1B9) [NBP3-07348]

Flow Cytometry: GTF2H2 Antibody (PCRP-GTF2H2-1B9) [NBP3-07348]

Flow Cytometry: GTF2H2 Antibody (PCRP-GTF2H2-1B9) [NBP3-07348] - Flow Cytometric Analysis of PFA-fixed HeLa cells. GTF2H2 Mouse Monoclonal Antibody (PCRP-GTF2H2-1B9) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).
Immunocytochemistry/ Immunofluorescence: GTF2H2 Antibody (PCRP-GTF2H2-1B9) [NBP3-07348]

Immunocytochemistry/ Immunofluorescence: GTF2H2 Antibody (PCRP-GTF2H2-1B9) [NBP3-07348]

Immunocytochemistry/Immunofluorescence: GTF2H2 Antibody (PCRP-GTF2H2-1B9) [NBP3-07348] - Immunofluorescence Analysis of PFA-fixed HeLa cells. GTF2H2 Mouse Monoclonal Antibody (PCRP-GTF2H2-1B9) followed by goat anti- Mouse IgG-CF488 (green). Phalloidin counterstain (red).
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Applications for GTF2H2 Antibody (PCRP-GTF2H2-1B9)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: GTF2H2

Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFII. TFIIH (or BTF2) is a multisubunit transcription/DNA repair factor that possesses several enzymatic activities. The core of TFIIH is composed of five subunits, designated p89 (XPB or ERCC3), p62, p52, p44 and p34. Additional subunits of the TFIIH complex are p80 (XPD or ERCC2) and the ternary kinase complex composed of Cdk7, cyclin H and MAT1. Both p89 and p80 have ATP-dependent helicase activity. The p62, p52 and p44 subunits have been shown to be involved in nucleotide excision repair.

Alternate Names

Basic transcription factor 2 44 kDa subunit, BTF2, BTF2 p44, BTF2P44TFIIH basal transcription factor complex p44 subunit, General transcription factor IIH polypeptide 2, general transcription factor IIH subunit 2, general transcription factor IIH, polypeptide 2 (44kD subunit), general transcription factor IIH, polypeptide 2, 44kD subunit, general transcription factor IIH, polypeptide 2, 44kDa, MGC102806, T-BTF2P44, TFIIH

Gene Symbol

GTF2H2

UniProt

Q13888 (Human)

Additional GTF2H2 Products

Product Documents for GTF2H2 Antibody (PCRP-GTF2H2-1B9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GTF2H2 Antibody (PCRP-GTF2H2-1B9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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