GTF3C5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49504

Novus Biologicals, part of Bio-Techne
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NBP2-49504
NBP2-49504-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GTF3C5 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: HREGYNNPPISGENLIGLSRARRPHNAIFVNFEDEEVPKQPLEAAAQTWRRVCTNPVDRKVEEELRKLFDIRPIWSRNA

Reactivity Notes

Mouse (86%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GTF3C5 Antibody

Western Blot: GTF3C5 Antibody [NBP2-49504]

Western Blot: GTF3C5 Antibody [NBP2-49504]

Western Blot: GTF3C5 Antibody [NBP2-49504] - Analysis using Anti-GTF3C5 antibody NBP2-49504 (A) shows similar pattern to independent antibody NBP2-55707 (B).
Immunocytochemistry/ Immunofluorescence: GTF3C5 Antibody [NBP2-49504]

Immunocytochemistry/ Immunofluorescence: GTF3C5 Antibody [NBP2-49504]

Immunocytochemistry/Immunofluorescence: GTF3C5 Antibody [NBP2-49504] - Staining of human cell line HaCaT shows localization to nucleoplasm.
Immunohistochemistry: GTF3C5 Antibody [NBP2-49504]

Immunohistochemistry: GTF3C5 Antibody [NBP2-49504]

Immunohistochemistry: GTF3C5 Antibody [NBP2-49504] - Staining of human duodenum shows strong nuclear positivity in glandular cells.

Applications for GTF3C5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GTF3C5

General transcription factor 3C polypeptide 5 (GTF3C5/TFIIIC63) is a subunit of the TFIIIC DNA-binding factor required for transcription of tRNA and 5S rRNA genes by RNA polymerase III. Human TFIIIC is composed of six subunits: TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, and TFIIIC35. GTF3C5/TFIIIC63 interacts with TFIIIB and may facilitate TFIIIB and RNA polymerase III recruitment in association with TFIIIC102, TFIIIB90, and RPC62.

Alternate Names

FLJ20857, general transcription factor 3C polypeptide 5, general transcription factor IIIC, polypeptide 5 (63kD), general transcription factor IIIC, polypeptide 5, 63kDa, TF3C-epsilon, TFiiiC2-63, TFIIIC63TFIIIC 63 kDa subunit, TFIIICepsilon, Transcription factor IIIC 63 kDa subunit, Transcription factor IIIC subunit epsilon, transcription factor IIIC, 63 kD

Gene Symbol

GTF3C5

Additional GTF3C5 Products

Product Documents for GTF3C5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GTF3C5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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