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GTPBP3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91974

Novus Biologicals, part of Bio-Techne
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NBP1-91974
NBP1-91974-25ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HALRILTAPRDLPLARHASLRLLSDPRSGEPLDRALVLWFPGPQSFTGEDCVEFHVHGGPAVVSGVLQALGSVPGLRPAEAGEFTRRAFANGKLNL

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25434004).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GTPBP3 Antibody

Western Blot: GTPBP3 Antibody [NBP1-91974]

Western Blot: GTPBP3 Antibody [NBP1-91974]

Western Blot: GTPBP3 Antibody [NBP1-91974] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: GTPBP3 Antibody [NBP1-91974]

Immunocytochemistry/ Immunofluorescence: GTPBP3 Antibody [NBP1-91974]

Immunocytochemistry/Immunofluorescence: GTPBP3 Antibody [NBP1-91974] - Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.
Immunohistochemistry-Paraffin: GTPBP3 Antibody [NBP1-91974]

Immunohistochemistry-Paraffin: GTPBP3 Antibody [NBP1-91974]

Immunohistochemistry-Paraffin: GTPBP3 Antibody [NBP1-91974] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.

Applications for GTPBP3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GTPBP3

GTPase involved in the 5-carboxymethylaminomethyl modification (mnm(5)s(2)U34) of the wobble uridine base inmitochondrial tRNAs (Probable)

Alternate Names

FLJ14700, FLJ17836, GTP binding protein 3 (mitochondrial), GTPBG3, GTP-binding protein 3, MSS1, MTGP1Mitochondrial GTP-binding protein 1, THDF1, tRNA modification GTPase GTPBP3, mitochondrial

Gene Symbol

GTPBP3

Additional GTPBP3 Products

Product Documents for GTPBP3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GTPBP3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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