Guanylin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89724

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Guanylin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SLESVKKLKDLQEPQEPRVGKLRNFAPIPGEPVVPILCSNPNFPEELKPLCKEPNAQEILQRLEEIAEDPGTCEICAYAACTGC

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25304930).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Guanylin Antibody

Immunohistochemistry-Paraffin: Guanylin Antibody [NBP1-89724]

Immunohistochemistry-Paraffin: Guanylin Antibody [NBP1-89724]

Immunohistochemistry-Paraffin: Guanylin Antibody [NBP1-89724] - Staining in human small intestine and liver tissues using anti-GUCA2A antibody. Corresponding GUCA2A RNA-seq data are presented for the same tissues.
Western Blot: Guanylin Antibody [NBP1-89724]

Western Blot: Guanylin Antibody [NBP1-89724]

Western Blot: Guanylin Antibody [NBP1-89724] - Analysis in control (vector only transfected HEK293T lysate) and gUCA2A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Guanylin Antibody [NBP1-89724]

Immunohistochemistry-Paraffin: Guanylin Antibody [NBP1-89724]

Immunohistochemistry-Paraffin: Guanylin Antibody [NBP1-89724] - Staining of human liver shows low expression as expected.
View 1 more images

Applications for Guanylin Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Guanylin

Endogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptorbinding region as the heat-stable enterotoxins

Alternate Names

GCAP-I, Guanylate cyclase activator 2A, guanylate cyclase activator 2A (guanylin), Guanylate cyclase-activating protein 1, Guanylate cyclase-activating protein I, guanylin, GUCA2guanylate cyclase activator 2A (guanylin 2, intestinal, heat-stable), STARA

Gene Symbol

GUCA2A

Additional Guanylin Products

Product Documents for Guanylin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Guanylin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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