Guanylin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89724
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SLESVKKLKDLQEPQEPRVGKLRNFAPIPGEPVVPILCSNPNFPEELKPLCKEPNAQEILQRLEEIAEDPGTCEICAYAACTGC
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 25304930).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Guanylin Antibody
Western Blot: Guanylin Antibody [NBP1-89724]
Western Blot: Guanylin Antibody [NBP1-89724] - Analysis in control (vector only transfected HEK293T lysate) and gUCA2A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunohistochemistry-Paraffin: Guanylin Antibody [NBP1-89724]
Immunohistochemistry-Paraffin: Guanylin Antibody [NBP1-89724] - Staining of human liver shows low expression as expected.Applications for Guanylin Antibody
Application
Recommended Usage
Immunohistochemistry
1:1000 - 1:2500
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Guanylin
Alternate Names
GCAP-I, Guanylate cyclase activator 2A, guanylate cyclase activator 2A (guanylin), Guanylate cyclase-activating protein 1, Guanylate cyclase-activating protein I, guanylin, GUCA2guanylate cyclase activator 2A (guanylin 2, intestinal, heat-stable), STARA
Gene Symbol
GUCA2A
Additional Guanylin Products
Product Documents for Guanylin Antibody
Product Specific Notices for Guanylin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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