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Guanylyl Cyclase alpha 1 Antibody (3G6B2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37401

Novus Biologicals, part of Bio-Techne
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NBP2-37401
NBP2-37401-0.025ml

Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3G6B2

Format

BSA Free

Concentration

1 mg/ml

Product Summary for Guanylyl Cyclase alpha 1 Antibody (3G6B2) - BSA Free

Immunogen

Purified recombinant fragment of human Guanylyl Cyclase alpha 1 (AA: 22-214) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

77.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Guanylyl Cyclase alpha 1 Antibody (3G6B2) - BSA Free

Western Blot: Guanylyl Cyclase alpha 1 Antibody (3G6B2)BSA Free [NBP2-37401]

Western Blot: Guanylyl Cyclase alpha 1 Antibody (3G6B2)BSA Free [NBP2-37401]

Western Blot: Guanylyl Cyclase alpha 1 Antibody (3G6B2) [NBP2-37401] - Western blot analysis using GUCY1A3 mouse mAb against HEK293 (1) and Raji (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: Guanylyl Cyclase alpha 1 Antibody (3G6B2) - BSA Free [NBP2-37401]

Immunocytochemistry/ Immunofluorescence: Guanylyl Cyclase alpha 1 Antibody (3G6B2) - BSA Free [NBP2-37401]

Immunocytochemistry/Immunofluorescence: Guanylyl Cyclase alpha 1 Antibody (3G6B2) [NBP2-37401] - Immunofluorescence analysis of HepG2 cells using GUCY1A3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: Guanylyl Cyclase alpha 1 Antibody (3G6B2) - BSA Free [NBP2-37401]

Immunohistochemistry: Guanylyl Cyclase alpha 1 Antibody (3G6B2) - BSA Free [NBP2-37401]

Immunohistochemistry: Guanylyl Cyclase alpha 1 Antibody (3G6B2) [NBP2-37401] - Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using GUCY1A3 mouse mAb with DAB staining.

Applications for Guanylyl Cyclase alpha 1 Antibody (3G6B2) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Guanylyl Cyclase alpha 1

Soluble guanylate cyclase (sGC), a heterodimeric protein consisting of an alpha subunit, such as alpha-1 (GUCY1A3), and a beta subunit, typically beta-1 (GUCY1B3; MIM 139397), catalyzes conversion of GTP to the second messenger cGMP and functions as the main receptor for nitric oxide and nitrovasodilator drugs (Zabel et al., 1998 [PubMed 9742212]).[supplied by OMIM]

Long Name

Guanylate cyclase soluble subunit alpha-1

Alternate Names

EC 4.6.1.2, GCS-alpha-1, GCS-alpha-3, GUC1A3, GUCSA3, GUCY1A1, GUCY1A3

Gene Symbol

GUCY1A1

Additional Guanylyl Cyclase alpha 1 Products

Product Documents for Guanylyl Cyclase alpha 1 Antibody (3G6B2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Guanylyl Cyclase alpha 1 Antibody (3G6B2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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