Guanylyl Cyclase alpha 1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35266
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Guanylyl Cyclase alpha 1 (NP_000847.2).
Sequence:
MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAE
Sequence:
MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAE
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Guanylyl Cyclase alpha 1 Antibody
Immunocytochemistry/ Immunofluorescence: Guanylyl Cyclase alpha 1 Antibody [NBP3-35266] -
Immunocytochemistry/ Immunofluorescence: Guanylyl Cyclase alpha 1 Antibody [NBP3-35266] - Immunofluorescence analysis of L929 cells using Guanylyl Cyclase alpha 1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western Blot: Guanylyl Cyclase alpha 1 Antibody [NBP3-35266] -
Western Blot: Guanylyl Cyclase alpha 1 Antibody [NBP3-35266] - Western blot analysis of various lysates using Guanylyl Cyclase alpha 1 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Applications for Guanylyl Cyclase alpha 1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:1000 - 1:3000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Guanylyl Cyclase alpha 1
Long Name
Guanylate cyclase soluble subunit alpha-1
Alternate Names
EC 4.6.1.2, GCS-alpha-1, GCS-alpha-3, GUC1A3, GUCSA3, GUCY1A1, GUCY1A3
Gene Symbol
GUCY1A1
Additional Guanylyl Cyclase alpha 1 Products
Product Documents for Guanylyl Cyclase alpha 1 Antibody
Product Specific Notices for Guanylyl Cyclase alpha 1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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