H1F0 Antibody (AE-4) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47753
![Flow Cytometry: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753]](https://resources.bio-techne.com/images/products/Histone-H1-Antibody-AE-4-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47753-img0001.jpg "Flow Cytometry: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753]")
![Western Blot: H1F0 Antibody (AE-4)Azide and BSA Free [NBP2-47753]](https://resources.bio-techne.com/images/products/Histone-H1-Antibody-AE-4-Azide-and-BSA-Free-Western-Blot-NBP2-47753-img0007.jpg "Western Blot: H1F0 Antibody (AE-4)Azide and BSA Free [NBP2-47753]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # AE-4
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for H1F0 Antibody (AE-4) - Azide and BSA Free
Immunogen
Nuclei of human leukemia biopsy cells
Localization
Nuclear
Marker
Pan Nuclear Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45182).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for H1F0 Antibody (AE-4) - Azide and BSA Free
Western Blot: H1F0 Antibody (AE-4)Azide and BSA Free [NBP2-47753]
Western Blot: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753] - Western Blot Analysis of human heart tissue lysate using H1F0 antibody (AE-4) - Azide and BSA Free![Immunocytochemistry/ Immunofluorescence: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753]](https://resources.bio-techne.com/images/products/Histone-H1-Antibody-AE-4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47753-img0008.jpg "Immunocytochemistry/ Immunofluorescence: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753]")
Immunocytochemistry/ Immunofluorescence: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753]
Immunocytochemistry/Immunofluorescence: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753] - Immunofluorescence staining of PFA-fixed HeLa cells using H1F0 antibody (AE-4) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (green). Membrane stained by Phalloidin![Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753]](https://resources.bio-techne.com/images/products/Histone-H1-Antibody-AE-4-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47753-img0006.jpg "Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753]")
Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753]
Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753] - Human Tonsil stained with H1F0 antibody (AE-4) - Azide and BSA Free![Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753]](https://resources.bio-techne.com/images/products/Histone-H1-Antibody-AE-4-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47753-img0003.jpg "Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753]")
![Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753]](https://resources.bio-techne.com/images/products/Histone-H1-Antibody-AE-4-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47753-img0005.jpg "Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) - Azide and BSA Free [NBP2-47753]")
Applications for H1F0 Antibody (AE-4) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
1mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: H1F0
Alternate Names
H1 histone family, member 0, H1.0, H1(0), H1-0, H10, H1FVMGC5241, Histone H1', Histone H1(0), histone H1.0
Gene Symbol
H1-0
Additional H1F0 Products
Product Documents for H1F0 Antibody (AE-4) - Azide and BSA Free
Product Specific Notices for H1F0 Antibody (AE-4) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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