H1F0 Antibody (SPM256) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11577

Novus Biologicals, part of Bio-Techne
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NBP3-11577
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # SPM256

Format

Azide and BSA Free

Concentration

1 mg/ml

View all H1F0 Antibodies »

Product Summary for H1F0 Antibody (SPM256) - Azide and BSA Free

Immunogen

Nuclei of human leukemia biopsy cells

Localization

Nuclear

Marker

Pan Nuclear Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45183).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for H1F0 Antibody (SPM256) - Azide and BSA Free

Western Blot: H1F0 Antibody (SPM256)Azide and BSA Free [NBP3-11577]

Western Blot: H1F0 Antibody (SPM256)Azide and BSA Free [NBP3-11577]

Western Blot: H1F0 Antibody (SPM256) - Azide and BSA Free [NBP3-11577] - Western Blot Analysis of human heart tissue lysate using H1F0 antibody (SPM256).
Immunocytochemistry/ Immunofluorescence: H1F0 Antibody (SPM256) - Azide and BSA Free [NBP3-11577]

Immunocytochemistry/ Immunofluorescence: H1F0 Antibody (SPM256) - Azide and BSA Free [NBP3-11577]

Immunocytochemistry/Immunofluorescence: H1F0 Antibody (SPM256) - Azide and BSA Free [NBP3-11577] - Confocal Immunofluorescence image of HeLa cells using H1F0 antibody (SPM256) (green-CF488). Phalloidin is used to label the membranes.

Applications for H1F0 Antibody (SPM256) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: H1F0

Plasmid: pCMV-H1F0 Full length; Host Cell: 293T

Alternate Names

H1 histone family, member 0, H1.0, H1(0), H1-0, H10, H1FVMGC5241, Histone H1', Histone H1(0), histone H1.0

Gene Symbol

H1-0

Additional H1F0 Products

Product Documents for H1F0 Antibody (SPM256) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for H1F0 Antibody (SPM256) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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