H1F0 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16772

Novus Biologicals, part of Bio-Techne
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NBP2-16772
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all H1F0 Antibodies »

Product Summary for H1F0 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human H1F0.0. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for H1F0 Antibody

Western Blot: H1F0 Antibody [NBP2-16772]

Western Blot: H1F0 Antibody [NBP2-16772]

Western Blot: H1F0 Antibody [NBP2-16772] - HepG2 whole cell extracts and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1 antibody (NBP2-16772) at a dilution of 1:10000 and developed with Trident Sharp-ECL.
Immunocytochemistry/ Immunofluorescence: H1F0 Antibody [NBP2-16772]

Immunocytochemistry/ Immunofluorescence: H1F0 Antibody [NBP2-16772]

Immunocytochemistry/Immunofluorescence: H1F0 Antibody [NBP2-16772] - Histone H1.0 antibody detects Histone H1.0 protein at nucleus by immunofluorescent analysis.Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H1.0 stained by Histone H1.0 antibody diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 um.
Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772]

Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772]

Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Paraffin-embedded mouse brain. Histone H1.0 antibody diluted at 1:50
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Applications for H1F0 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: H1F0

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H1 family. [provided by RefSeq]

Alternate Names

H1 histone family, member 0, H1.0, H1(0), H1-0, H10, H1FVMGC5241, Histone H1', Histone H1(0), histone H1.0

Gene Symbol

H1-0

Additional H1F0 Products

Product Documents for H1F0 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for H1F0 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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