H1FX Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-29518

Novus Biologicals, part of Bio-Techne
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NBP3-29518
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all H1FX Antibodies »

Product Specifications

Immunogen

Between 163 and 213

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for H1FX Antibody

H1FX Antibody

Immunoprecipitation:H1FX AntibodyNBP3-29518] -

Immunoprecipitation:H1FX AntibodyNBP3-29518] -Detection of human Histone H1x by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Histone H1x antibody NBP3-29518 (lot NBP3-29518-1) used for IP at 6 µg per reaction. Histone H1x was also immunoprecipitated by rabbit anti-Histone H1x.. For blotting immunoprecipitated Histone H1x, A304-603A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for H1FX Antibody

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate buffer, pH 7 to 8

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: H1FX

Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber as they play a critical role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. The H1FX gene encodes a H1 Histone family, member X protein that is 213 amino acids long at 22 kDA. H1 Histones are critical for condensation of nucleosome chains into higher-order structures. H1FX participates in PKA signaling, Granzyme-A pathway, histone modification, and cell cycle start of DNA replication in early S phase. H1FX is known to interact with XBP1, TOP1, RRP1B, NPM1, and DOT1L. The H1FX gene was associated with treacher collins syndrome and metastasis efficiency.

Alternate Names

H1 histone family, member X, H1X, histone H1x, MGC15959, MGC8350

Gene Symbol

H1-10

Additional H1FX Products

Product Documents for H1FX Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for H1FX Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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