H4 Clustered Histone 1 [ac Lys5] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-37888
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Chromatin Immunoprecipitation, ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic acetylated peptide around K5 of human H4 Clustered Histone 1 (NP_001029249.1).
Sequence:
GRGKG
Sequence:
GRGKG
Modification
ac Lys 5
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for H4 Clustered Histone 1 [ac Lys5] Antibody
![H4 Clustered Histone 1 [ac Lys5] Antibody](https://resources.bio-techne.com/images/products/nbp3-37888_rabbit-h4-clustered-histone-1-ac-lys-5-pab-91220241418117.jpg "Chromatin Immunoprecipitation: H4 Clustered Histone 1 [ac Lys5] Antibody [NBP3-37888] -")
Chromatin Immunoprecipitation: H4 Clustered Histone 1 [ac Lys5] Antibody [NBP3-37888] -
Chromatin Immunoprecipitation: H4 Clustered Histone 1 [ac Lys5] Antibody [NBP3-37888] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using H4 Clustered Histone 1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.![H4 Clustered Histone 1 [ac Lys5] Antibody](https://resources.bio-techne.com/images/products/nbp3-37888_rabbit-h4-clustered-histone-1-ac-lys-5-pab-91220241418165.jpg "Western Blot: H4 Clustered Histone 1 [ac Lys5] Antibody [NBP3-37888] -")
Western Blot: H4 Clustered Histone 1 [ac Lys5] Antibody [NBP3-37888] -
Western Blot: H4 Clustered Histone 1 [ac Lys5] Antibody [NBP3-37888] - Western blot analysis of various lysates using H4 Clustered Histone 1 Rabbit pAb at 1:1000 dilution. HeLa cells,NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37C for 18 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Applications for H4 Clustered Histone 1 [ac Lys5] Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation
5ug antibody for 5ug-10ug of Chromatin
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: H4 Clustered Histone 1
Alternate Names
H4-16;H4C11;H4C12;H4C13;H4C14;H4C15;H4C2;H4C3;H4C4;H4C5;H4C6;H4C8;H4C9;H4FA, H4F4, HIST1H4A
Gene Symbol
H4C1
Additional H4 Clustered Histone 1 Products
Product Documents for H4 Clustered Histone 1 [ac Lys5] Antibody
Product Specific Notices for H4 Clustered Histone 1 [ac Lys5] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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