H4 Clustered Histone 1 [Monomethyl Lys20] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-37992
![H4 Clustered Histone 1 [Monomethyl Lys20] Antibody](https://resources.bio-techne.com/images/products/nbp3-37992_rabbit-h4-clustered-histone-1-monomethyl-lys-20-pab-912202415111815.jpg "Immunohistochemistry: H4 Clustered Histone 1 [Monomethyl Lys20] Antibody [NBP3-37992] -")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Dot Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic monomethylated peptide around K20 of human H4 Clustered Histone 1 (NP_003529.1).
Sequence:
HRKVL
Sequence:
HRKVL
Modification
Monomethyl Lys 20
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for H4 Clustered Histone 1 [Monomethyl Lys20] Antibody
![H4 Clustered Histone 1 [Monomethyl Lys20] Antibody](https://resources.bio-techne.com/images/products/nbp3-37992_rabbit-h4-clustered-histone-1-monomethyl-lys-20-pab-912202415175262.jpg "Dot Blot: H4 Clustered Histone 1 [Monomethyl Lys20] Antibody [NBP3-37992] -")
Dot Blot: H4 Clustered Histone 1 [Monomethyl Lys20] Antibody [NBP3-37992] -
Dot Blot: H4 Clustered Histone 1 [Monomethyl Lys20] Antibody [NBP3-37992] - Dot-blot analysis of all sorts of methylation peptides using H4 Clustered Histone 1 antibody.![H4 Clustered Histone 1 [Monomethyl Lys20] Antibody](https://resources.bio-techne.com/images/products/nbp3-37992_rabbit-h4-clustered-histone-1-monomethyl-lys-20-pab-912202414154391.jpg "Western Blot: H4 Clustered Histone 1 [Monomethyl Lys20] Antibody [NBP3-37992] -")
Western Blot: H4 Clustered Histone 1 [Monomethyl Lys20] Antibody [NBP3-37992] -
Western Blot: H4 Clustered Histone 1 [Monomethyl Lys20] Antibody [NBP3-37992] - Western blot analysis of various lysates using H4 Clustered Histone 1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
![H4 Clustered Histone 1 [Monomethyl Lys20] Antibody](https://resources.bio-techne.com/images/products/nbp3-37992_rabbit-h4-clustered-histone-1-monomethyl-lys-20-pab-912202414201393.jpg "Immunocytochemistry/ Immunofluorescence: H4 Clustered Histone 1 [Monomethyl Lys20] Antibody [NBP3-37992] -")
Immunocytochemistry/ Immunofluorescence: H4 Clustered Histone 1 [Monomethyl Lys20] Antibody [NBP3-37992] -
Immunocytochemistry/ Immunofluorescence: H4 Clustered Histone 1 [Monomethyl Lys20] Antibody [NBP3-37992] - Immunofluorescence analysis of 293T cells using H4 Clustered Histone 1 Rabbit pAb. Blue: DAPI for nuclear staining.![H4 Clustered Histone 1 [Monomethyl Lys20] Antibody](https://resources.bio-techne.com/images/products/nbp3-37992_rabbit-h4-clustered-histone-1-monomethyl-lys-20-pab-912202415111835.jpg "Immunohistochemistry: H4 Clustered Histone 1 [Monomethyl Lys20] Antibody [NBP3-37992] -")
![H4 Clustered Histone 1 [Monomethyl Lys20] Antibody](https://resources.bio-techne.com/images/products/nbp3-37992_rabbit-h4-clustered-histone-1-monomethyl-lys-20-pab-912202414201331.jpg "Immunohistochemistry: H4 Clustered Histone 1 [Monomethyl Lys20] Antibody [NBP3-37992] -")
![H4 Clustered Histone 1 [Monomethyl Lys20] Antibody](https://resources.bio-techne.com/images/products/nbp3-37992_rabbit-h4-clustered-histone-1-monomethyl-lys-20-pab-912202415111885.jpg "Western Blot: H4 Clustered Histone 1 [Monomethyl Lys20] Antibody [NBP3-37992] -")
Applications for H4 Clustered Histone 1 [Monomethyl Lys20] Antibody
Application
Recommended Usage
Dot Blot
1:500 - 1:2000
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: H4 Clustered Histone 1
Alternate Names
H4-16;H4C11;H4C12;H4C13;H4C14;H4C15;H4C2;H4C3;H4C4;H4C5;H4C6;H4C8;H4C9;H4FA, H4F4, HIST1H4A
Gene Symbol
H4C1
Additional H4 Clustered Histone 1 Products
Product Documents for H4 Clustered Histone 1 [Monomethyl Lys20] Antibody
Product Specific Notices for H4 Clustered Histone 1 [Monomethyl Lys20] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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