HADHB Antibody

Western Blot: HADHB Antibody [NBP1-82609]

Western Blot: HADHB Antibody [NBP1-82609] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry-Paraffin: HADHB Antibody [NBP1-82609]

Immunohistochemistry-Paraffin: HADHB Antibody [NBP1-82609] - Staining of human heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.

Immunohistochemistry-Paraffin: HADHB Antibody [NBP1-82609]

Immunohistochemistry-Paraffin: HADHB Antibody [NBP1-82609] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: HADHB Antibody [NBP1-82609]

Immunohistochemistry-Paraffin: HADHB Antibody [NBP1-82609] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: HADHB Antibody [NBP1-82609]

Immunohistochemistry-Paraffin: HADHB Antibody [NBP1-82609] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.

Western Blot: HADHB Antibody [NBP1-82609] -

Western Blot: HADHB Antibody [NBP1-82609] - Identification of Proteins Binding Pre-miR-329 & Pre-miR-495(A) Schematic representation of RNA pull-down combined with SILAC mass spectrometry. (B) Proteins showing increased or decreased binding to pre-miR-329, pre-miR-495, or both. (C) Western blot validation of HADHB & CIRBP binding to pre-miR-329 & pre-miR-495 under conditions of normal serum & serum starvation. DHX9 is used as a binding control. (D & E) Quantification of the western blot, showing HADHB (D) & CIRBP (E) binding to pre-miR-329 & pre-miR-495 under conditions of normal serum & serum starvation (relative to input). *p < 0.05, n = 3. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30665182), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HADHB Antibody [NBP1-82609] -

Western Blot: HADHB Antibody [NBP1-82609] - Generation of NIH 3T3 HADHB KO Cell Lines by CRISPR/Cas9(A) Western blot analysis of HADHB protein levels in two HADHB KO clones (AQ & Z) compared with decreasing amounts of total protein extract from WT NIH 3T3 cells. (B) Alignment of the region surrounding the CRISPR-Cas9 target sequence from genomic DNA of clones AQ & Z. (C & D) Levels of mature (C) or precursor (D) miR-329 & miR-495 as well as control miR-423 in WT & HADHB KO cell lines quantified by qRT-PCR & normalized to miR-16. For pre-miRs, n = 3; for mature miRs, n = 9. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30665182), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HADHB Antibody [NBP1-82609] -

Western Blot: HADHB Antibody [NBP1-82609] - Expression of CIRBP & HADHB In Vivo(A & B) Immunohistochemical staining of murine adductor muscle after ischemia induction revealed expression of both CIRBP (A) & HADHB (B) in these tissues, predominantly in the cytoplasm of cells. (C & D) Microarray analysis of mRNA expression of CIRBP (C) & HADHB (D) mRNA in the adductor muscle of mice at several time points after induction of ischemia (4 mice per time point). (E) Western blot showing HADHB levels in murine adductor muscle tissue on day 0, day 1, & day 3 after hindlimb ligation. For each time point, samples from right (R) unligated paws & left (L) ligated paws are presented next to each other. Tubulin was used as a loading control. (F) Quantification of the western blot. The HADHB signal was normalized against tubulin, & the relative change between ligated & unligated is presented. Each bar represents a biological triplicate & technical duplicate. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30665182), licensed under a CC-BY license. Not internally tested by Novus Biologicals.