Haptoglobin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48880
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Haptoglobin Antibody
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: GKPKNPANPVQRILGGHLDAKGSFPWQAKMVSHHNLTTGATLINEQWLLTTAKNLFLNHSENATAKDIAPTLTLYVG
Reactivity Notes
Mouse (81%), Rat (83%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Haptoglobin Antibody
Western Blot: Haptoglobin Antibody [NBP2-48880]
Western Blot: Haptoglobin Antibody [NBP2-48880] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HepG2
Immunocytochemistry/ Immunofluorescence: Haptoglobin Antibody [NBP2-48880]
Immunocytochemistry/Immunofluorescence: Haptoglobin Antibody [NBP2-48880] - Staining of human cell line Hep G2 shows localization to nucleoplasm & vesicles.Immunohistochemistry-Paraffin: Haptoglobin Antibody [NBP2-48880]
Immunohistochemistry-Paraffin: Haptoglobin Antibody [NBP2-48880] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.Applications for Haptoglobin Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Haptoglobin
Alternate Names
HP, HP-1, hp2-alpha, HPA1S
Gene Symbol
HP
Additional Haptoglobin Products
Product Documents for Haptoglobin Antibody
Product Specific Notices for Haptoglobin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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