HARS2 Antibody (OTI5H2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71739

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Canine, Monkey, Primate

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI5H2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all HARS2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HARS2(NP_036340) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

53.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HARS2 Antibody (OTI5H2) - Azide and BSA Free

Western Blot: HARS2 Antibody (OTI5H2)Azide and BSA Free [NBP2-71739]

Western Blot: HARS2 Antibody (OTI5H2)Azide and BSA Free [NBP2-71739]

Western Blot: HARS2 Antibody (OTI5H2) - Azide and BSA Free [NBP2-71739] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HARS2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HARS2.
Immunohistochemistry: HARS2 Antibody (OTI5H2) - Azide and BSA Free [NBP2-71739]

Immunohistochemistry: HARS2 Antibody (OTI5H2) - Azide and BSA Free [NBP2-71739]

Immunohistochemistry: HARS2 Antibody (OTI5H2) - Azide and BSA Free [NBP2-71739] - Staining of paraffin-embedded Human Ovary tissue using anti-HARS2 mouse monoclonal antibody.
Western Blot: HARS2 Antibody (OTI5H2)Azide and BSA Free [NBP2-71739]

Western Blot: HARS2 Antibody (OTI5H2)Azide and BSA Free [NBP2-71739]

Western Blot: HARS2 Antibody (OTI5H2) - Azide and BSA Free [NBP2-71739] - Analysis of extracts (35ug) from 9 different cell lines by using anti-HARS2 monoclonal antibody.
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Applications for HARS2 Antibody (OTI5H2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HARS2

The HARS2 gene encodes a 506 amino acid, 56kDA probable histidine--tRNA ligase (mitochondrial) protein that is member to the II family of aminoacyl-tRNA synthetases which charge tRNA with cognate amino acids. The HARS2 gene is located in a head-to-head orientation with HARS on chromosome 5, which is the location of where homologous genes share a bidirectional promoter. The protein works as an accessory in the regulation of protein biosynthesis through the synthesis of histidyl-transfer RNA. HARS2 is involved in gene expression and mitochondrian tRNA aminoacylation and has been known to interact with genes ICT1, AARS2, AASS, ABSB7, and ACAD9. HARS2 has been investigated in immunodeficiency, malaria, pneumonia, hearing loss, tuberculosis, schizophrenia, and ovarian dysgenesis.

Alternate Names

EC 6.1.1, EC 6.1.1.21, HARSLprobable histidyl-tRNA synthetase, mitochondrial, HARS-related, HARSRhistidine tRNA ligase 2, mitochondrial (putative), hisRS, histidine translase, Histidine--tRNA ligase, Histidine--tRNA ligase-like, histidyl-tRNA synthetase 2, mitochondrial (putative), histidyl-tRNA synthetase-like, HO3histidine-tRNA ligase homolog

Gene Symbol

HARS2

Additional HARS2 Products

Product Documents for HARS2 Antibody (OTI5H2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HARS2 Antibody (OTI5H2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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