HAUS8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77746

Novus Biologicals, part of Bio-Techne
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NBP1-77746
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit Serum

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HAUS8 Antibodies »

Product Specifications

Immunogen

HAUS8 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant full length HAUS8 protein. (Uniprot: Q9BT25)

Reactivity Notes

A BLAST analysis was used to suggest reactivity with HAUS8 from human based on a 100% homology with the immunizing sequence. Expect reactivity with HAUS8 from chimpanzee, Sumatran orangutan based on a 90% homology with the immunizing sequence. Cross-reactivity with HAUS8 from other sources has not been determined.

Specificity

This antibody reacts with endogenous HAUS8 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

Serum

Description

This product was adsorbed against GST from monospecific antiserum by immunoaffinity chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for HAUS8 Antibody

Western Blot: HAUS8 Antibody [NBP1-77746]

Western Blot: HAUS8 Antibody [NBP1-77746]

Western Blot: HAUS8 Antibody [NBP1-77746] - Analysis shows detection of a 45 kDa band corresponding to endogenous HICE1 in lysates of S phase HeLa cells silenced for either control Luciferase or HAUS8. In right lane (HAUS8): lysates from sh-HAUS8 RNAi-treated lentivirus-infected cells. In left lane (GLi): lysates from sh-Luciferase lentivirus-infected cells as control. Anti-HICE1 Antibody was used at 1:10,000. Molecular weight estimation was made by comparison by prestained MW markers. ECL was used for detection.

Applications for HAUS8 Antibody

Application
Recommended Usage

ELISA

1:250000

Western Blot

1:10000
Application Notes
This antiserum has been tested for use in ELISA and western blotting using a full length recombinant Hice1 protein. Specific conditions for reactivity and detection of Hice1 should be optimized by the end user. Expect a band approximately ~45 kDa in size corresponding to Hice1 by Western Blotting in the appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: HAUS8

HAUS8 contributes to the mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Normal bipolar spindle formation is critical for accurate chromosome segregation and proper mitotic progression. Failure in this event leads to spindle checkpoint activation and chromosome missegregation that ultimately leads to aneuploidy. Hice1 binds to microtubules directly, and promotes spindle integrity and chromosome stability. Hice1 has also shown to play an important role in targeting the 'TuRC complex to the mitotic spindle, a step that appears to be required for spindle-mediated microtubule generation and normal chromosome segregation. The HAUS augmin-like complex's interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. During interphase, it is primarily cytoplasmic, associating with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, it additionally associates with the spindle midzone and midbody, respectively. Further characterization of the function of Hice1 will likely be important for better understanding the mechanism of normal mitotic progression and high fidelity chromosome segregation.

Alternate Names

DGT4, HAUS Augmin Like Complex Subunit 8, HEC1/NDC80 interacting, centrosome associated 1, HEC1/NDC80-Interacting Centrosome-Associated Protein 1, Hec1-Interacting And Centrosome-Associated 1, HICE1, NY-SAR-48, Sarcoma Antigen NY-SAR-48

Gene Symbol

HAUS8

UniProt

Q9BT25 (Human), NP_219485 (Human)

Additional HAUS8 Products

Product Documents for HAUS8 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HAUS8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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