HBG1 Antibody (6F1A8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15299
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 6F1A8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for HBG1 Antibody (6F1A8)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HBG1 (P69891). MGHFTEEDKATITSLWGKVNVEDAGGETLGRLLVVYPWTQRFFDSFGNLSSASAIMGNPKVKAHGKKVLTSLGDAIKHLDDLKGTFAQLSELHCDKLHVD
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HBG1 Antibody (6F1A8)
Western Blot: HBG1 Antibody (6F1A8) [NBP3-15299]
Western Blot: HBG1 Antibody (6F1A8) [NBP3-15299] - Western blot analysis of extracts of Mouse kidney cells, using HBG1 antibody (NBP3-15299) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Immunohistochemistry: HBG1 Antibody (6F1A8) [NBP3-15299]
Immunohistochemistry: HBG1 Antibody (6F1A8) [NBP3-15299] - Immunofluorescence analysis of human placenta using HBG1 Rabbit mAb (NBP3-15299) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: HBG1 Antibody (6F1A8) [NBP3-15299]
Immunohistochemistry-Paraffin: HBG1 Antibody (6F1A8) [NBP3-15299] - Immunohistochemistry of paraffin-embedded human placenta using HBG1 Rabbit mAb (NBP3-15299) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for HBG1 Antibody (6F1A8)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HBG1
Alternate Names
gamma-1-globin, Hb F Agamma, HBGA, HBGR, HBG-T2, Hemoglobin gamma A, Hemoglobin gamma-1 chain, Hemoglobin gamma-A chain, Hemoglobin subunit gamma 1, HSGGL1, PRO2979
Gene Symbol
HBG1
Additional HBG1 Products
Product Documents for HBG1 Antibody (6F1A8)
Product Specific Notices for HBG1 Antibody (6F1A8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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