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HBO1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92161

Novus Biologicals, part of Bio-Techne
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NBP1-92161
NBP1-92161-25ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GCNSLGHLTGKHERHFSISGCPLYHNLSADECKAPTERQLRYKEKVAELRKKRNSGLSKEQKEKYMEHRQTYGNTREPLLENLTSEYDLDLFRRAQARASEDLEKLRLQGQITEGSNM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HBO1 Antibody

Western Blot: HBO1 Antibody [NBP1-92161]

Western Blot: HBO1 Antibody [NBP1-92161]

Western Blot: HBO1 Antibody [NBP1-92161] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: HBO1 Antibody [NBP1-92161]

Immunocytochemistry/ Immunofluorescence: HBO1 Antibody [NBP1-92161]

Immunocytochemistry/Immunofluorescence: HBO1 Antibody [NBP1-92161] - Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: HBO1 Antibody [NBP1-92161]

Immunohistochemistry-Paraffin: HBO1 Antibody [NBP1-92161]

Immunohistochemistry-Paraffin: HBO1 Antibody [NBP1-92161] - Staining of human stomach shows moderate nuclear positivity in glandular cells.

Applications for HBO1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HBO1

HBO1 (human acetylase binding to ORC1) is a MYST family histone acetylase. HBO1 has shown to be targeted to origins of replication in association with the licensing factor Cdt1 and to also interact with transcriptional activator proteins.

Long Name

Histone acetyltransferase KAT7

Alternate Names

EC 2.3.1.48, HBOa, KAT7, MYST-2, MYST2

Entrez Gene IDs

11143 (Human); 217127 (Mouse); 303470 (Rat)

Gene Symbol

KAT7

UniProt

Additional HBO1 Products

Product Documents for HBO1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HBO1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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