HCA59 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83169
Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: YHEELNAPIRRNKEEPKARPLRVGDTEKPEPERSPPNRKRPANEKATDDYHYEKFK
Reactivity Notes
Reactivity reported in scientific literature (PMID: 23276153)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HCA59 Antibody
Western Blot: HCA59 Antibody [NBP1-83169] -
Western Blot: HCA59 Antibody [NBP1-83169] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probes #1 and #2, using Anti-C9orf78 antibody. The remaining relative intensity is presented.
Immunohistochemistry: HCA59 Antibody [NBP1-83169] -
Immunohistochemistry: HCA59 Antibody [NBP1-83169] - Staining of human bone marrow, fallopian tube, lymph node and testis using Anti-C9orf78 antibody NBP1-83169 (A) shows similar protein distribution across tissues to independent antibody NBP1-83170 (B).
Immunohistochemistry: HCA59 Antibody [NBP1-83169] -
Immunohistochemistry: HCA59 Antibody [NBP1-83169] - Staining of human pancreas shows weak positivity in exocrine glandular cells as expected.
Applications for HCA59 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
1.0 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HCA59
Alternate Names
bA409K20.3, C9orf78, chromosome 9 open reading frame 78, HCA59, Hepatocellular carcinoma-associated antigen 59, HSPC220
Gene Symbol
C9ORF78
Additional HCA59 Products
Product Documents for HCA59 Antibody
Product Specific Notices for HCA59 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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