HCLS1 Antibody (1A8)

Novus Biologicals, part of Bio-Techne | Catalog # H00003059-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Proximity Ligation Assay, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1A8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HCLS1 Antibodies »

Product Specifications

Immunogen

HCLS1 (AAH16758, 266 a.a. ~ 355 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QERKAVTKRSPEAPQPVIAMEEPAVPAPLPKKISSEAWPPVGTPPSSESEPVRTSREHPVPLLPIRQTLPEDNEEPPALPPRTLEGLQVE

Specificity

HCLS1 (1A8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for HCLS1 Antibody (1A8)

Western Blot: HCLS1 Antibody (1A8) [H00003059-M02]

Western Blot: HCLS1 Antibody (1A8) [H00003059-M02]

Western Blot: HCLS1 Antibody (1A8) [H00003059-M02] - Analysis of HCLS1 expression in transfected 293T cell line by HCLS1 monoclonal antibody (M02), clone 1A8.Lane 1: HCLS1 transfected lysate(54 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: HCLS1 Antibody (1A8) [H00003059-M02]

Immunohistochemistry-Paraffin: HCLS1 Antibody (1A8) [H00003059-M02]

Immunohistochemistry-Paraffin: HCLS1 Antibody (1A8) [H00003059-M02] - Analysis of monoclonal antibody to HCLS1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
Western Blot: HCLS1 Antibody (1A8) [H00003059-M02]

Western Blot: HCLS1 Antibody (1A8) [H00003059-M02]

Western Blot: HCLS1 Antibody (1A8) [H00003059-M02] - Analysis of HCLS1 over-expressed 293 cell line, cotransfected with HCLS1 Validated Chimera RNAi ( Cat # H00003059-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HCLS1 monoclonal antibody (M02), clone 1A8 (Cat # H00003059-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
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Applications for HCLS1 Antibody (1A8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HCLS1

Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling forboth clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression

Long Name

Hematopoietic Cell-specific Lyn Substrate 1

Alternate Names

Cortactin-like, CTTNL, LckBP1

Entrez Gene IDs

3059 (Human)

Gene Symbol

HCLS1

OMIM

601306 (Human)

UniProt

AAH16758 (Human)

Additional HCLS1 Products

Product Documents for HCLS1 Antibody (1A8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HCLS1 Antibody (1A8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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