HDAC1 Antibody (PCRP-HDAC1-1B7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13936

Novus Biologicals, part of Bio-Techne
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NBP3-13936-100ug
NBP3-13936-20ug
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # PCRP-HDAC1-1B7

Concentration

0.2 mg/ml

View all Histone Deacetylase 1/HDAC1 Antibodies »

Product Summary for HDAC1 Antibody (PCRP-HDAC1-1B7)

Immunogen

Recombinant full-length human HDAC1 protein (Uniprot: Q13547)

Localization

Nucleus, Nucleoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14062)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for HDAC1 Antibody (PCRP-HDAC1-1B7)

Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936]

Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936]

Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HDAC1 Antibody (PCRP-HDAC1-1B7) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).
Flow Cytometry: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936]

Flow Cytometry: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936]

Flow Cytometry: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936] - Flow cytometric analysis of PFA-fixed HeLa cells. HDAC1 antibody (PCRP-HDAC1-1B7) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Protein Array: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936]

Protein Array: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936]

Protein Array: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HDAC1 Antibody (PCRP-HDAC1-1B7).

Applications for HDAC1 Antibody (PCRP-HDAC1-1B7)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein
Application Notes
ELISA: For coating, order antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Histone Deacetylase 1/HDAC1

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Long Name

Histone Deacetylase 1

Alternate Names

GON-10, HD1, KDAC1, RPD3L1

Gene Symbol

HDAC1

UniProt

Q13547 (Human)

Additional Histone Deacetylase 1/HDAC1 Products

Product Documents for HDAC1 Antibody (PCRP-HDAC1-1B7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HDAC1 Antibody (PCRP-HDAC1-1B7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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