HDAC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56340

Novus Biologicals, part of Bio-Techne
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NB100-56340
NB100-56340SS
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockout Validated, Simple Western, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockdown Validated, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Histone Deacetylase 1/HDAC1 Antibodies »

Product Specifications

Immunogen

This antibody was generated by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 1-5, 433-448 and 467-482 of human HDAC1 (Genbank Accession no. Q13547).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25648995). Rat reactivity reported in scientific literature (PMID: 26551542)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HDAC1 Antibody

Knockout Validated: HDAC1 Antibody [NB100-56340]

Knockout Validated: HDAC1 Antibody [NB100-56340]

Knockout Validated: HDAC1 Antibody [NB100-56340] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HDAC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human HDAC1 Polyclonal Antibody (Catalog # NB100-56340) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 65 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: HDAC1 Antibody [NB100-56340]

Western Blot: HDAC1 Antibody [NB100-56340]

Western Blot: HDAC1 Antibody [NB100-56340] - Analysis of HDAC1 in HEK293 cell lysate with this antibody.
Immunohistochemistry-Paraffin: HDAC1 Antibody [NB100-56340]

Immunohistochemistry-Paraffin: HDAC1 Antibody [NB100-56340]

Immunohistochemistry-Paraffin: HDAC1 Antibody [NB100-56340] - Human testis using HDAC1 antibody at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.
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Applications for HDAC1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500. Use reported in multiple pieces of scientific literature

Immunohistochemistry-Frozen

1:10-1:500. Use reported in scientific literature (PMID 26551542)

Immunohistochemistry-Paraffin

1:10-1:500. Use reported in scientific literature (PMID 25648995)

Simple Western

1:200

Western Blot

2 ug/ml
Application Notes
In 293, a 60 kDa band is observed. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.

Reviewed Applications

Read 2 reviews rated 4.5 using NB100-56340 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 1/HDAC1

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

Long Name

Histone Deacetylase 1

Alternate Names

GON-10, HD1, KDAC1, RPD3L1

Entrez Gene IDs

3065 (Human)

Gene Symbol

HDAC1

UniProt

Q01101 (Human)

Additional Histone Deacetylase 1/HDAC1 Products

Product Documents for HDAC1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HDAC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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