HDAC6 Antibody (9V1W2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16082

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16082-100ul
NBP3-16082-20ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9V1W2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone Deacetylase 6/HDAC6 Antibodies »

Product Summary for HDAC6 Antibody (9V1W2)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HDAC6 (Q9UBN7). MTSTGQDSTTTRQRRSRQNPQSPPQDSSVTSKRNIKKGAVPRSIPNLAEVKKKGKMKKLGQAMEEDLIVGLQGMDLNLEAEALAGTGLVLDEQLNEFHCL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HDAC6 Antibody (9V1W2)

Western Blot: HDAC6 Antibody (9V1W2) [NBP3-16082]

Western Blot: HDAC6 Antibody (9V1W2) [NBP3-16082]

Western Blot: HDAC6 Antibody (9V1W2) [NBP3-16082] - Western blot analysis of extracts of various cell lines, using HDAC6 antibody (NBP3-16082) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: HDAC6 Antibody (9V1W2) [NBP3-16082]

Immunohistochemistry-Paraffin: HDAC6 Antibody (9V1W2) [NBP3-16082]

Immunohistochemistry-Paraffin: HDAC6 Antibody (9V1W2) [NBP3-16082] - Immunohistochemistry of paraffin-embedded human breast cancer using HDAC6 Rabbit mAb (NBP3-16082) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunoprecipitation: HDAC6 Antibody (9V1W2) [NBP3-16082]

Immunoprecipitation: HDAC6 Antibody (9V1W2) [NBP3-16082]

Immunoprecipitation: HDAC6 Antibody (9V1W2) [NBP3-16082] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug HDAC6 antibody (NBP3-16082). Western blot was performed from the immunoprecipitate using HDAC6 antibody (NBP3-16082) at a dilition of 1:1000.

Applications for HDAC6 Antibody (9V1W2)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 6/HDAC6

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.

Long Name

Histone Deacetylase 6

Alternate Names

CPBHM, FLJ16239, HD6, HDAC6, JM21, PPP1R90

Gene Symbol

HDAC6

Additional Histone Deacetylase 6/HDAC6 Products

Product Documents for HDAC6 Antibody (9V1W2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HDAC6 Antibody (9V1W2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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