HDAC6 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56343

Novus Biologicals, part of Bio-Techne
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NB100-56343
NB100-56343SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Histone Deacetylase 6/HDAC6 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an N-terminal portion of human HDAC6 (between amino acids 1-50) [UniProt Q9UBN7]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HDAC6 Antibody - BSA Free

Western Blot: HDAC6 AntibodyBSA Free [NB100-56343]

Western Blot: HDAC6 AntibodyBSA Free [NB100-56343]

Western Blot: HDAC6 Antibody [NB100-56343] - Analysis of HDAC-6 in NIH-3T3 cell lysate with this antibody.
Immunocytochemistry/ Immunofluorescence: HDAC6 Antibody - BSA Free [NB100-56343]

Immunocytochemistry/ Immunofluorescence: HDAC6 Antibody - BSA Free [NB100-56343]

Immunocytochemistry/Immunofluorescence: HDAC6 Antibody [NB100-56343] - HeLa cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-HDAC6 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Simple Western: HDAC6 AntibodyBSA Free [NB100-56343]

Simple Western: HDAC6 AntibodyBSA Free [NB100-56343]

Simple Western: HDAC6 Antibody [NB100-56343] - Lane view shows a specific band for HDAC6 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for HDAC6 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

1:20-1:1000. Use reported in scientific literature (Imbriano (2005))

Chromatin Immunoprecipitation (ChIP)

1:20-1:1000

Immunocytochemistry/ Immunofluorescence

2 - 5 ug/ml

Immunoprecipitation

assay dependent

Simple Western

1:20

Western Blot

2 - 5 ug/ml
Application Notes
In NIH 3T3, a 134 kDa band is observed. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 6/HDAC6

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

Long Name

Histone Deacetylase 6

Alternate Names

CPBHM, FLJ16239, HD6, HDAC6, JM21, PPP1R90

Entrez Gene IDs

10013 (Human); 15185 (Mouse)

Gene Symbol

HDAC6

Additional Histone Deacetylase 6/HDAC6 Products

Product Documents for HDAC6 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HDAC6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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